PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains

Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been availab...

Full description

Saved in:
Bibliographic Details
Published in:Medical mycology (Oxford) 1998-01, Vol.36 (2), p.61-67
Main Authors: Wedde, M., Müller, D., Tintelnot, K., de Hoog, G.S., Stahl, U.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953
cites cdi_FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953
container_end_page 67
container_issue 2
container_start_page 61
container_title Medical mycology (Oxford)
container_volume 36
creator Wedde, M.
Müller, D.
Tintelnot, K.
de Hoog, G.S.
Stahl, U.
description Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.
doi_str_mv 10.1080/02681219880000111
format article
fullrecord <record><control><sourceid>oup_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_02681219880000111</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1080/02681219880000111</oup_id><sourcerecordid>10.1080/02681219880000111</sourcerecordid><originalsourceid>FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953</originalsourceid><addsrcrecordid>eNqNkNtKAzEQhoMoWKsP4N0-gGtz2CYTvJLiCQoWtddLNgeask1Ksiv07Y3UO0Gcm5l_-L-B-RG6JviWYMAzTDkQSiQALkUIOUET0nBcU4HlaZkZlzUTwM_RRc7bYhGSsglarxZvdaeyNZU3Ngzeea0GH0MVXaV7H4rs-0OVbG8_VRiqVbajKSub9cYmr2bv2pqY9zH5cVflISkf8iU6c6rP9uqnT9H68eFj8VwvX59eFvfLWlPeDHWDwUgDVhAgHLpGgQDHDDDDxLxjTkqgc840bYpSyklLO8mJ0KAwd3LOpogc7-oUc07WtfvkdyodWoLb71zaX7kU5ubIxHH_L_vd0e6Di2mnNlb1w0arZNttHFMo7_1BfwHtVHT3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains</title><source>Oxford Journals Online</source><creator>Wedde, M. ; Müller, D. ; Tintelnot, K. ; de Hoog, G.S. ; Stahl, U.</creator><creatorcontrib>Wedde, M. ; Müller, D. ; Tintelnot, K. ; de Hoog, G.S. ; Stahl, U.</creatorcontrib><description>Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.1080/02681219880000111</identifier><language>eng</language><publisher>UK: Informa UK Ltd</publisher><ispartof>Medical mycology (Oxford), 1998-01, Vol.36 (2), p.61-67</ispartof><rights>1998 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1998</rights><rights>1998 International Society for Human and Animal Mycology 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953</citedby><cites>FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Wedde, M.</creatorcontrib><creatorcontrib>Müller, D.</creatorcontrib><creatorcontrib>Tintelnot, K.</creatorcontrib><creatorcontrib>de Hoog, G.S.</creatorcontrib><creatorcontrib>Stahl, U.</creatorcontrib><title>PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains</title><title>Medical mycology (Oxford)</title><description>Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.</description><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqNkNtKAzEQhoMoWKsP4N0-gGtz2CYTvJLiCQoWtddLNgeask1Ksiv07Y3UO0Gcm5l_-L-B-RG6JviWYMAzTDkQSiQALkUIOUET0nBcU4HlaZkZlzUTwM_RRc7bYhGSsglarxZvdaeyNZU3Ngzeea0GH0MVXaV7H4rs-0OVbG8_VRiqVbajKSub9cYmr2bv2pqY9zH5cVflISkf8iU6c6rP9uqnT9H68eFj8VwvX59eFvfLWlPeDHWDwUgDVhAgHLpGgQDHDDDDxLxjTkqgc840bYpSyklLO8mJ0KAwd3LOpogc7-oUc07WtfvkdyodWoLb71zaX7kU5ubIxHH_L_vd0e6Di2mnNlb1w0arZNttHFMo7_1BfwHtVHT3</recordid><startdate>199801</startdate><enddate>199801</enddate><creator>Wedde, M.</creator><creator>Müller, D.</creator><creator>Tintelnot, K.</creator><creator>de Hoog, G.S.</creator><creator>Stahl, U.</creator><general>Informa UK Ltd</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199801</creationdate><title>PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains</title><author>Wedde, M. ; Müller, D. ; Tintelnot, K. ; de Hoog, G.S. ; Stahl, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wedde, M.</creatorcontrib><creatorcontrib>Müller, D.</creatorcontrib><creatorcontrib>Tintelnot, K.</creatorcontrib><creatorcontrib>de Hoog, G.S.</creatorcontrib><creatorcontrib>Stahl, U.</creatorcontrib><collection>CrossRef</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wedde, M.</au><au>Müller, D.</au><au>Tintelnot, K.</au><au>de Hoog, G.S.</au><au>Stahl, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains</atitle><jtitle>Medical mycology (Oxford)</jtitle><date>1998-01</date><risdate>1998</risdate><volume>36</volume><issue>2</issue><spage>61</spage><epage>67</epage><pages>61-67</pages><issn>1369-3786</issn><eissn>1460-2709</eissn><abstract>Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.</abstract><cop>UK</cop><pub>Informa UK Ltd</pub><doi>10.1080/02681219880000111</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1369-3786
ispartof Medical mycology (Oxford), 1998-01, Vol.36 (2), p.61-67
issn 1369-3786
1460-2709
language eng
recordid cdi_crossref_primary_10_1080_02681219880000111
source Oxford Journals Online
title PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T04%3A26%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-oup_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PCR-based%20identification%20of%20clinically%20relevant%20Pseudallescheria/Scedosporium%20strains&rft.jtitle=Medical%20mycology%20(Oxford)&rft.au=Wedde,%20M.&rft.date=1998-01&rft.volume=36&rft.issue=2&rft.spage=61&rft.epage=67&rft.pages=61-67&rft.issn=1369-3786&rft.eissn=1460-2709&rft_id=info:doi/10.1080/02681219880000111&rft_dat=%3Coup_cross%3E10.1080/02681219880000111%3C/oup_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c264t-408d9d8e718168b4a878f3d83d375b3f9982563c245b3aaf9e2b9617c8a06f953%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_oup_id=10.1080/02681219880000111&rfr_iscdi=true