A Novel Vector System for Gene Transfer into the Cornea Using a Partially Dried Plasmid Expressing 18 Basic Fibroblast Growth Factor-Synthetic Amphiphile INTeraction-18 (SAINT-18) Complex

Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 4...

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Bibliographic Details
Published in:Current eye research 2008-01, Vol.33 (10), p.839-848
Main Authors: Kuo, Chien-Neng, Yang, Lin-Cheng, Yang, Cheng-Ta, Chen, Miao-Fen, Lai, Chien-Hsiung, Chen, Yi-Hao, Chen, Ching-Hsein, Chen, Chi-Hung, Wu, Pei-Chang, Kou, Hsi-Kung, Tsai, Jen-Chia, Hung, Chia-Hui
Format: Article
Language:English
Subjects:
angiogenesis
Animals
Blotting, Western
cornea
Corneal Neovascularization - genetics
Corneal Neovascularization - pathology
Corneal Stroma - metabolism
fibroblast growth factor
Fibroblast Growth Factor 2 - genetics
Gene Expression - physiology
Gene Transfer Techniques
Genetic Vectors
Immunohistochemistry
Male
nonviral vector
Plasmids - genetics
Pyridinium Compounds - chemistry
Rats
Rats, Sprague-Dawley
SAINT
Surface-Active Agents
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Summary:Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μ g of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μ g, 2 μ g, or 20 μ g of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μ g p-bFGF group, days 12-18 in the 2-μ g p-bFGF group, and on days 9-15 in the 0.2-μ g p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.
ISSN:0271-3683
1460-2202
DOI:10.1080/02713680802382963