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The Effect of Sildenafil on Selenite-Induced Cataract in Rats
To investigate the effect of sildenafil on an experimental sodium selenite-induced cataract model in rats. Twenty-six young Wistar rats were separated into four groups. On postpartum day 10, six rats received only selenite (group 1, selenite-induced cataract), seven rats received selenite and high d...
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Published in: | Current eye research 2020-09, Vol.45 (9), p.1082-1088 |
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creator | Atalay, Hatice Tuba Ucgul, Ahmet Yucel Turkcu, Ummuhani Ozel Ozmen, Mehmet Cuneyt Yilmaz, Samet Bilgihan, Ayse |
description | To investigate the effect of sildenafil on an experimental sodium selenite-induced cataract model in rats.
Twenty-six young Wistar rats were separated into four groups. On postpartum day 10, six rats received only selenite (group 1, selenite-induced cataract), seven rats received selenite and high dose oral sildenafil (group 2, high-dose sildenafil-treated), seven rats received selenite and low dose oral sildenafil (group 3, low-dose sildenafil-treated), and six rats received only saline (group 4, controls). On postpartum day 30, cataract formation was graded and recorded using an operating microscope. The rats were sacrificed, lens tissues were isolated, and serum samples were collected. Nitrite oxide metabolites (NOx), advanced oxidative protein products (AOPP), and total sulfhydryl (TSH) levels were assessed in both serum and lenticular samples.
The rats treated with low-dose sildenafil showed lower levels of AOPP and NOx, and the higher levels of TSH than the rats in other experimental groups. Otherwise, the rats treated with high-dose sildenafil, similar to the selenite-induced cataract group, showed higher levels of AOPP and serum NOx than rats in the low-dose sildenafil-treated group. The rats treated with low-dose sildenafil also showed less cataract development than rats in the other experimental groups.
Low doses (0.7 mg/kg) of oral sildenafil might show a protective effect on cataract development by lowering oxidative stress. |
doi_str_mv | 10.1080/02713683.2020.1726405 |
format | article |
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Twenty-six young Wistar rats were separated into four groups. On postpartum day 10, six rats received only selenite (group 1, selenite-induced cataract), seven rats received selenite and high dose oral sildenafil (group 2, high-dose sildenafil-treated), seven rats received selenite and low dose oral sildenafil (group 3, low-dose sildenafil-treated), and six rats received only saline (group 4, controls). On postpartum day 30, cataract formation was graded and recorded using an operating microscope. The rats were sacrificed, lens tissues were isolated, and serum samples were collected. Nitrite oxide metabolites (NOx), advanced oxidative protein products (AOPP), and total sulfhydryl (TSH) levels were assessed in both serum and lenticular samples.
The rats treated with low-dose sildenafil showed lower levels of AOPP and NOx, and the higher levels of TSH than the rats in other experimental groups. Otherwise, the rats treated with high-dose sildenafil, similar to the selenite-induced cataract group, showed higher levels of AOPP and serum NOx than rats in the low-dose sildenafil-treated group. The rats treated with low-dose sildenafil also showed less cataract development than rats in the other experimental groups.
Low doses (0.7 mg/kg) of oral sildenafil might show a protective effect on cataract development by lowering oxidative stress.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.1080/02713683.2020.1726405</identifier><identifier>PMID: 32023416</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>AOPP ; cataract model ; NOx ; selenite ; sildenafil ; total sulfhydryl</subject><ispartof>Current eye research, 2020-09, Vol.45 (9), p.1082-1088</ispartof><rights>2020 Taylor & Francis Group, LLC 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-e5402478e504e6ac9d96023bdd1a84c4b22b27094de91456a8d755d27a1b05003</citedby><cites>FETCH-LOGICAL-c333t-e5402478e504e6ac9d96023bdd1a84c4b22b27094de91456a8d755d27a1b05003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32023416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atalay, Hatice Tuba</creatorcontrib><creatorcontrib>Ucgul, Ahmet Yucel</creatorcontrib><creatorcontrib>Turkcu, Ummuhani Ozel</creatorcontrib><creatorcontrib>Ozmen, Mehmet Cuneyt</creatorcontrib><creatorcontrib>Yilmaz, Samet</creatorcontrib><creatorcontrib>Bilgihan, Ayse</creatorcontrib><title>The Effect of Sildenafil on Selenite-Induced Cataract in Rats</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>To investigate the effect of sildenafil on an experimental sodium selenite-induced cataract model in rats.
Twenty-six young Wistar rats were separated into four groups. On postpartum day 10, six rats received only selenite (group 1, selenite-induced cataract), seven rats received selenite and high dose oral sildenafil (group 2, high-dose sildenafil-treated), seven rats received selenite and low dose oral sildenafil (group 3, low-dose sildenafil-treated), and six rats received only saline (group 4, controls). On postpartum day 30, cataract formation was graded and recorded using an operating microscope. The rats were sacrificed, lens tissues were isolated, and serum samples were collected. Nitrite oxide metabolites (NOx), advanced oxidative protein products (AOPP), and total sulfhydryl (TSH) levels were assessed in both serum and lenticular samples.
The rats treated with low-dose sildenafil showed lower levels of AOPP and NOx, and the higher levels of TSH than the rats in other experimental groups. Otherwise, the rats treated with high-dose sildenafil, similar to the selenite-induced cataract group, showed higher levels of AOPP and serum NOx than rats in the low-dose sildenafil-treated group. The rats treated with low-dose sildenafil also showed less cataract development than rats in the other experimental groups.
Low doses (0.7 mg/kg) of oral sildenafil might show a protective effect on cataract development by lowering oxidative stress.</description><subject>AOPP</subject><subject>cataract model</subject><subject>NOx</subject><subject>selenite</subject><subject>sildenafil</subject><subject>total sulfhydryl</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kNtKw0AQhhdRbK0-grIvkDp7yuFCUErVQkGw9XqZZHcxkm7Kbor07ZvQ1kuvBv75_jn8hNwzmDLI4RF4xkSaiykH3ksZTyWoCzJmMoWE9-IlGQ9MMkAjchPjD8AgyGsyEn1fSJaOydP629K5c7bqaOvoqm6M9ejqhraermxjfd3ZZOHNrrKGzrDDgD1ae_qJXbwlVw6baO9OdUK-Xufr2Xuy_HhbzF6WSSWE6BKrJHCZ5VaBtClWhSnS_oDSGIa5rGTJeckzKKSxBZMqxdxkShmeIStBAYgJUce5VWhjDNbpbag3GPaagR7i0Oc49BCHPsXR-x6Ovu2u3Fjz5zr_3wPPR6D2rg0b_G1DY3SH-6YNLqCv6qjF_zsOrjxsOw</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Atalay, Hatice Tuba</creator><creator>Ucgul, Ahmet Yucel</creator><creator>Turkcu, Ummuhani Ozel</creator><creator>Ozmen, Mehmet Cuneyt</creator><creator>Yilmaz, Samet</creator><creator>Bilgihan, Ayse</creator><general>Taylor & Francis</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200901</creationdate><title>The Effect of Sildenafil on Selenite-Induced Cataract in Rats</title><author>Atalay, Hatice Tuba ; Ucgul, Ahmet Yucel ; Turkcu, Ummuhani Ozel ; Ozmen, Mehmet Cuneyt ; Yilmaz, Samet ; Bilgihan, Ayse</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-e5402478e504e6ac9d96023bdd1a84c4b22b27094de91456a8d755d27a1b05003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>AOPP</topic><topic>cataract model</topic><topic>NOx</topic><topic>selenite</topic><topic>sildenafil</topic><topic>total sulfhydryl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atalay, Hatice Tuba</creatorcontrib><creatorcontrib>Ucgul, Ahmet Yucel</creatorcontrib><creatorcontrib>Turkcu, Ummuhani Ozel</creatorcontrib><creatorcontrib>Ozmen, Mehmet Cuneyt</creatorcontrib><creatorcontrib>Yilmaz, Samet</creatorcontrib><creatorcontrib>Bilgihan, Ayse</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atalay, Hatice Tuba</au><au>Ucgul, Ahmet Yucel</au><au>Turkcu, Ummuhani Ozel</au><au>Ozmen, Mehmet Cuneyt</au><au>Yilmaz, Samet</au><au>Bilgihan, Ayse</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Sildenafil on Selenite-Induced Cataract in Rats</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>45</volume><issue>9</issue><spage>1082</spage><epage>1088</epage><pages>1082-1088</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><abstract>To investigate the effect of sildenafil on an experimental sodium selenite-induced cataract model in rats.
Twenty-six young Wistar rats were separated into four groups. On postpartum day 10, six rats received only selenite (group 1, selenite-induced cataract), seven rats received selenite and high dose oral sildenafil (group 2, high-dose sildenafil-treated), seven rats received selenite and low dose oral sildenafil (group 3, low-dose sildenafil-treated), and six rats received only saline (group 4, controls). On postpartum day 30, cataract formation was graded and recorded using an operating microscope. The rats were sacrificed, lens tissues were isolated, and serum samples were collected. Nitrite oxide metabolites (NOx), advanced oxidative protein products (AOPP), and total sulfhydryl (TSH) levels were assessed in both serum and lenticular samples.
The rats treated with low-dose sildenafil showed lower levels of AOPP and NOx, and the higher levels of TSH than the rats in other experimental groups. Otherwise, the rats treated with high-dose sildenafil, similar to the selenite-induced cataract group, showed higher levels of AOPP and serum NOx than rats in the low-dose sildenafil-treated group. The rats treated with low-dose sildenafil also showed less cataract development than rats in the other experimental groups.
Low doses (0.7 mg/kg) of oral sildenafil might show a protective effect on cataract development by lowering oxidative stress.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>32023416</pmid><doi>10.1080/02713683.2020.1726405</doi><tpages>7</tpages></addata></record> |
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subjects | AOPP cataract model NOx selenite sildenafil total sulfhydryl |
title | The Effect of Sildenafil on Selenite-Induced Cataract in Rats |
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