Residues and dissipation of the pesticide emamectin benzoate under Egyptian field condition: a case study

A simple, rapid analytical procedure for the quantification of emamectin benzoate in tomato was developed using high performance liquid chromatography with fluorescence detector (HPLC-FLD). Emamectin benzoate residues were extracted from tomato samples with n-hexane:ethyl acetate (1:1, v/v). The met...

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Bibliographic Details
Published in:Toxicological and environmental chemistry 2013-08, Vol.95 (7), p.1099-1107
Main Authors: Malhat, Farag, Fayz, Abd El-Salam, Loutfy, Naglaa M., Ahmed, Mohamed Tawfic
Format: Article
Language:English
Subjects:
Acetates
Anhydrides
Benzoates
Chromatography
Crop residues
Detectors
Dissipation
Dosage
emamectin benzoate
Fluorescence
food safety
High performance liquid chromatography
Lycopersicon esculentum
pesticide residue
Pesticides
Residues
tomato
Tomatoes
Toxicology
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Summary:A simple, rapid analytical procedure for the quantification of emamectin benzoate in tomato was developed using high performance liquid chromatography with fluorescence detector (HPLC-FLD). Emamectin benzoate residues were extracted from tomato samples with n-hexane:ethyl acetate (1:1, v/v). The method involved derivatization using trifluoroacetic anhydride and 1-methylimidazole, prior to analysis by HPLC-FLD. Limit of quantification of this method was found to be 0.01 mg/kg, while limit of detection was 0.005 mg/kg. The dissipation of emamectin benzoate on tomato was studied, and half-life (t 1/2 ) was estimated in a field study carried out at three different locations. Emamectin benzoate was sprayed on tomato at recommended dosage. Samples of tomato were collected at 0, 1, 3, 7, 10, and 15 days after treatment. The t 1/2 of emamectin benzoate when applied at the recommended doses in tomato was approximately 2.5 days. The residue of emamectin benzoate reached below the maximal residue limit (MRL) 10 days after spraying at the tested dosage. Hence, a safe pre-harvest interval of 10 days is suggested before harvesting of tomato. This study would also be helpful for the Egypt government to establish the MRL of emamectin benzoate in tomatoes and provide guidance on the proper and safe use of this insecticide.
ISSN:0277-2248
1029-0486
DOI:10.1080/02772248.2013.865908