Parasitological and molecular survey of scattered parasitism by trichomonads in some avian species in Iran

• Published in: Avian pathology 2020-01, Vol.49 (1), p.47-55
• Main Authors: Arabkhazaeli, F., Madani, S. A., Ghorbani, A.
• Format: Article
• Language: English
• Subjects: Animals; Anseriformes; Anseriformes - parasitology; avian; Bird Diseases - epidemiology; Bird Diseases - parasitology; Body organs; Canaries - parasitology; Cloaca; Columbiformes; Columbiformes - parasitology; Conjunctiva; Crop; Crows - parasitology; DNA, Protozoan - chemistry; DNA, Protozoan - isolation & purification; DNA, Ribosomal Spacer - chemistry; Esophagus; Falconiformes; Falconiformes - parasitology; Flagellates; Galliformes; Galliformes - parasitology; Gastrointestinal system; Gastrointestinal tract; Gene sequencing; Genetic diversity; Genetic variability; Genetic variation; Genotype; Genotype & phenotype; Genotypes; Humans; Identification; Intestine; Investigations; Iran - epidemiology; ITS genotypes; Melopsittacus - parasitology; Multilocus Sequence Typing - veterinary; Oesophagus; Organs; Parasites; Parasitism; Passeriformes; Passeriformes - parasitology; Phylogenetics; Phylogeny; Prevalence; Protozoa; Protozoan Infections, Animal - epidemiology; Protozoan Infections, Animal - parasitology; Psittaciformes - parasitology; RNA, Ribosomal, 5.8S - genetics; rRNA; rRNA 5.8S; Species; Species diversity; Starlings - parasitology; Surveying; Tetratrichomonas gallinarum; Trichomonadida; Trichomonadida - classification; Trichomonadida - genetics; Trichomonas - genetics; Trichomonas gallinae; trichomonosis; Virulence; Waterfowl
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079457.2019.1662369

Outbreaks of avian trichomonosis are being reported worldwide; meanwhile, the genetic and virulence variations are under investigation. In this study, the occurrence and genetic variability of oral or faecal trichomonads among various avian species were investigated. Samples obtained from either the oropharyngeal cavity, crop/oesophagus, droppings/cloaca, or conjunctival swabs of avian species were inspected for flagellates. Phylogenetic analysis of partial ITS1-5.8s rRNA-ITS2 sequences from selected samples was performed to investigate the genetic diversity of the isolates. Investigation of 737 birds revealed an infection rate of 15.7% in the upper gastrointestinal tract, 7.3% in the faecal samples, and 0.7% involvement of the conjunctiva. Phylogenetic analysis of partial ITS1-5.8s rRNA-ITS2 sequences from selected samples, identified genotypes A and B of Trichomonas gallinae and genogroups A-C and E of Tetratrichomonas gallinarum. A novel ITS genotype of intestinal trichomonads was also detected in hooded crow (Corvus cornix) and common mynah (Acridotheres tristis). In the present study, in addition to Columbiformes and Falconiformes, trichomonads were detected in Passeriformes and Galliformes with the involvement of organs other than the gastrointestinal tract. Genotype A T. gallinae was detected in domestic pigeons (Columba livia domestica), a laughing dove (Spilopelia senegalensis), a common kestrel (Falco tinnunculus), a budgerigar (Melopsittacus undulates), and a canary (Serinus canaria). Distinct genotype B was detected in a common mynah and a budgerigar. Genogroups A-C of T. gallinarum were also demonstrated in Galliformes and Anseriformes. Furthermore, two novel trichomonad ITS genotypes were detected in hooded crows and a common mynah warranting detailed multi-locus molecular analysis. RESEARCH HIGHLIGHTS ITS diversity of trichomonads was shown in various avian species. Diversity of the parasites' target organ and clinical manifestations was demonstrated. Two novel ITS genotype trichomonads from common mynah and hooded crow were identified.