Infected and apoptotic cells in the IBDV-infected bursa of Fabricius, studied by double-labelling techniques

Infections of young chickens with infectious bursal disease virus (IBDV) result in depletion of lymphoid cells of the bursa of Fabricius (BF) due to necrosis and apoptotic processes. Interactions between IBDV and lymphoid cells were investigated by labelling paraffin-embedded tissue sections of infe...

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Bibliographic Details
Published in:Avian pathology 1999-06, Vol.28 (3), p.279-285
Main Authors: Nieper, H, Teifke, J.P, Jungmann, A, Lohr, C.V, Muller, H
Format: Article
Language:English
Subjects:
antigen detection
apoptosis
assays
bursa of Fabricius
chickens
Deoxyribonucleic acid
Disease
DNA
immunohistochemistry
in situ tunel reaction
infectious bursal disease
Infectious bursal disease virus
nucleic acid hybridization
Pathology
Poultry
viral antigens
Viruses
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Summary:Infections of young chickens with infectious bursal disease virus (IBDV) result in depletion of lymphoid cells of the bursa of Fabricius (BF) due to necrosis and apoptotic processes. Interactions between IBDV and lymphoid cells were investigated by labelling paraffin-embedded tissue sections of infected BF with combinations of either immunohistochemistry (IHC), in situ hybridization (ISH) or in situ TUNEL reaction (IST). With regard to specificity and sensitivity, results of ISH were comparable to those of IHC. By double-labelling it was shown, for the first time, that viral antigen was present in most of the apoptotic cells. This suggests that IBDV may be directly involved in the induction of the apoptotic process. However, some cells also showed either viral antigen or DNA fragmentation, especially at the early stages of infection. It should be taken into account, therefore, that the apoptotic processes might also be induced by IBDV through indirect interaction between cells. Remarkably, in some of the infected lymphoid cells ISH signals were observed in the nucleolus.
ISSN:0307-9457
1465-3338
DOI:10.1080/03079459994777