A voltammetric screening method to determine ronidazole in bovine meat

An original voltammetric screening method, employing glassy carbon electrode (GCE) with the differential-pulse voltammetry technique (DPV), has been developed to determine residues of the anti-parasitic agent Ronidazole (RNZ) in bovine meat. By using cyclic voltammetry (CV), it has been demonstrated...

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Bibliographic Details
Published in:Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Pesticides, food contaminants, and agricultural wastes, 2020-06, Vol.55 (6), p.583-591
Main Authors: Diniz, Juliana Aparecida, Okumura, Leonardo Luiz, Aleixo, Herbert, Gurgel, Alexandre, Silva, Astréa Filomena Souza
Format: Article
Language:English
Subjects:
Antiparasitic agent
Antiparasitic agents
Differential pulse voltammetry
electroanalytical techniques
Electrodes
Electron transfer
Glassy carbon
glassy carbon electrode
Mass transfer
Meat
Metronidazole
pH effects
Potassium chloride
Screening
Silver chloride
Voltammetry
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Summary:An original voltammetric screening method, employing glassy carbon electrode (GCE) with the differential-pulse voltammetry technique (DPV), has been developed to determine residues of the anti-parasitic agent Ronidazole (RNZ) in bovine meat. By using cyclic voltammetry (CV), it has been demonstrated that an irreversible cathodic process occurs at approximately −0.740 V (vs. Ag|AgCl, KCl 3 mol L −1 ) in a 0.100 mol L −1 phosphate buffer at pH 6.5 as supporting electrolyte. Furthermore, the behavior of RNZ in CV indicates the occurrence of a diffusion mass transfer process to the working electrode surface. The RNZ reduction mechanism was proposed as a 6-electron transfer, similar to Metronidazole under the same pH range. Quantification of RNZ and method validation were then carried out by DPV. The relative standard deviation (RSD) were 3.21% for intraday precision of 10 consecutive repetitions and 6.78% for interday precision after five analysis. Limits of detection and quantification were also obtained, and the values were 0.107 and 0.358 mg kg −1 , respectively. The recovery percentage for three different concentrations of RNZ in the bovine meat matrix ranged between 98.1% and 100.3%. The method proved to be efficient for screening RNZ in bovine meat.
ISSN:0360-1234
1532-4109
DOI:10.1080/03601234.2020.1745523