Mechanistic studies on the drug metabolism and toxicity originating from cytochromes P450

• Published in: Drug metabolism reviews 2020-07, Vol.52 (3), p.366-394
• Main Authors: Jaladanki, Chaitanya K., Gahlawat, Anuj, Rathod, Gajanan, Sandhu, Hardeep, Jahan, Kousar, Bharatam, Prasad V.
• Format: Article
• Language: English
• Subjects: density functional theory; mass spectrometry; oxidative cyclization; Quantum chemistry; reactive metabolites; sight of metabolism
• ISSN: 0360-2532; 1097-9883
• DOI: 10.1080/03602532.2020.1765792

Cytochromes P450 are oxidizing enzymes; a few families of cytochromes P450 are implicated in drug metabolism. These enzymatic reactions involve many processes including (i) prodrug to drug conversion, (ii) easy excretion of drug, (iii) generation of reactive metabolites, many of which cause toxicity. In this review, the fundamental biochemical mechanisms associated with the conversion of drugs into the useful or toxic metabolites have been discussed. The mechanisms can be established with the help of many experimental methods like mass spectral analysis, NMR and in vitro analysis etc. Computational methods provide detailed atomic level information, which is generally not available from experimental studies. Thus, the in silico efforts in elucidating the molecular mechanisms are complementary to the known experimental methods and are often clearer (especially in providing 3D information about the metabolites and their reactions). Quantum chemical methods and molecular docking become especially very useful. This review includes five case studies, which explain how the atomic level details were obtained to explore the reaction mechanisms of drug metabolism by cytochromes P450.