distinct pathotype of Ralstonia (Pseudomonas) solanacearum race 1, biovar 1 entering Florida in pothos (Epipremnum aureum) cuttings

Ralstonia solanacearum was isolated from diseased pothos (Epipremnum aureum) cuttings imported to Florida from Costa Rica. Epipremnum aureum has not previously been described as a host of R. solanacearum. Bacterial isolates were identified by standard bacteriological tests, FAME analysis (MIDI), met...

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Bibliographic Details
Published in:Canadian journal of plant pathology 1998-06, Vol.20 (2), p.171-175
Main Authors: Norman, D.J, Yuen, J.M.F
Format: Article
Language:English
Subjects:
chemical composition
Epipremnum pinnatum
host range
identification
introduced species
molecular weight
Musa acuminata
new host records
pathogenicity
pathotypes
plant pathogenic bacteria
polymerase chain reaction
Ralstonia solanacearum
Solanum lycopersicum var. lycopersicum
Solanum tuberosum
strain differences
symptoms
virulence
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Summary:Ralstonia solanacearum was isolated from diseased pothos (Epipremnum aureum) cuttings imported to Florida from Costa Rica. Epipremnum aureum has not previously been described as a host of R. solanacearum. Bacterial isolates were identified by standard bacteriological tests, FAME analysis (MIDI), metabolic profile (Biolog), and by PCR using species-specific primers. All isolates were biovar 1, Gram-negative, oxidase negative, catalase positive, motile, strictly aerobic, and accumulated poly-beta-hydroxybutyrate. Similarity indices using MIDI matched in most cases to R. solanacearum; however, low similarity indices were obtained with the Biolog data base. PCR amplification and electrophoretic analysis revealed a 148 bp product for all pothos isolates, identical to that in R. solanacearum control strains. Pathogenicity of isolates from pothos were compared with tomato and potato isolates from Florida on tomato, potato, pothos, tobacco, and triploid banana. Isolates from pothos caused wilt of tomato and potato, variable symptoms on tobacco, and mild symptoms on banana; they were identified as race 1. Ralstonia solanacearum isolates from pothos represent a distinct pathotype of race 1, biovar 1 and differ from other race 1, biovar 1 strains by being pathogenic on pothos.
ISSN:0706-0661
1715-2992
DOI:10.1080/07060669809500423