Antioxidant, anti-quorum sensing, biofilm inhibitory activities and chemical composition of Patchouli essential oil: in vitro and in silico approach

The interest in naturally occurring essential oils from medicinal plants has increased extremely over the last decade markedly because they possess antimicrobial and antioxidant protective properties against different chronic diseases. Extensive survival of drug-resistant infectious bacteria depends...

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Published in:Journal of biomolecular structure & dynamics 2022, Vol.40 (1), p.154-165
Main Authors: Mansuri, Afrin, Lokhande, Kiran, Kore, Supriya, Gaikwad, Swapnil, Nawani, Neelu, Swamy, K. Venkateswara, Junnarkar, Manisha, Pawar, Sarika
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
anti-biofilm
anti-oxidant
Antioxidants - pharmacology
Biofilms
GC-MS analysis
molecular docking
Molecular Docking Simulation
Oils, Volatile - pharmacology
Patchouli essential oil
Plant Extracts - pharmacology
Pogostemon
Pogostemon cablin
Quorum Sensing
quorum sensing inhibition
Virulence Factors
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Summary:The interest in naturally occurring essential oils from medicinal plants has increased extremely over the last decade markedly because they possess antimicrobial and antioxidant protective properties against different chronic diseases. Extensive survival of drug-resistant infectious bacteria depends on quorum sensing (QS) signaling network which raises the need for alternative antibacterial compounds. The aim of this study was to examine the phytochemical compounds of patchouli essential oil (PEO) and to assess its antioxidant activity. Antioxidant studies estimated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method showed that the PEO has effective antioxidant activity (IC 50 19.53 µg/mL). QS inhibitory activity of PEO was examined by employing the biosensor strain, Chromobacterium violaceum CV12472. At sub-lethal concentrations, PEO potentially reduced the QS regulated violacein synthesis in CV12472 without inhibiting its cell proliferation. Moreover, it also effectively reduced the production of some QS regulated virulence factors and biofilm development in P. aeruginosa PAO1 without hindering its growth. Phytochemical analysis of PEO was done by GC/MS technique. Molecular docking of PEO major compounds with QS (LasR and FabI) and biofilm regulator proteins (MvfR and Sialidase) of PAO1 was evaluated. These phytocompounds showed potential hydrogen binding interactions with these proteins. The overall results, in vitro and in silico, suggest that PEO could be applied as biocontrol agent against antibiotic resistance pathogens. Communicated by Ramaswamy H. Sarma
ISSN:0739-1102
1538-0254
DOI:10.1080/07391102.2020.1810124