Reventilation with room air or 100% oxygen after asphyxia differentially affects cerebral neuropathology in newborn pigs

• Published in: Acta Paediatrica 2006-09, Vol.95 (9), p.1109-1115
• Main Authors: Domoki, Ferenc, Zimmermann, Aliz, Cserni, Gabor, Bori, Rita, Temesvári, Peter, Bari, Ferenc
• Format: Article
• Language: English
• Subjects: Air; Animals; Animals, Newborn; Asphyxia - blood; Asphyxia - pathology; Asphyxia - therapy; Biological and medical sciences; Blood Gas Analysis; Blood Pressure; Brain - blood supply; Brain - pathology; Cerebrovascular Circulation; Female; General aspects; Male; Medical sciences; Oxygen - adverse effects; Oxygen - therapeutic use; Oxygen toxicity; perinatal asphyxia; perinatal brain damage; piglet; Pulmonary Ventilation; Swine; Ventilation
• ISSN: 0803-5253; 1651-2227
• DOI: 10.1080/08035250600717139

Aim: To test if reventilation with room air (RA) or 100% oxygen (O2) after asphyxia would differentially affect neuronal damage in different brain areas of newborn pigs. Methods: Anaesthetized piglets were subjected to 10 min asphyxia (n= 27) or served as time controls (n= 7). Reventilation started with either RA or O2 for 1 h, and was continued with RA for an additional 1 – 3 h. Cortical or cerebellar blood flow was assessed with laser‐Doppler flowmetry (LDF). Haematoxylin/eosin‐stained sections from six brain regions were prepared for blinded neuropathological examination and scoring. Results: Asphyxia resulted in significant neuronal damage compared to time controls in all areas examined except the pons. O2 ventilation elicited greater neuronal lesions in the hippocampus and the cerebellum but smaller damage in the basal ganglia compared to RA. The assessed physiological parameters including the LDF signals were similar in both ventilation groups, except for PaO2 in the first hour of reventilation (RA 75±5 mmHg, O2 348±57 mmHg; p