A Duplex PCR Assay for Sex Determination of Cattle Meat by Simultaneous Amplification of SRY, AMELX and AMELY Genes

Analysis of the sex of cattle meat by fast and reliable molecular techniques is an important measure to ensure correct allocation of export refunds, particularly in European countries and also in countries such as India where female cattle (cow) slaughter is banned legally in many areas because of r...

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Bibliographic Details
Published in:Food biotechnology 2012-01, Vol.26 (1), p.75-84
Main Authors: Gokulakrishnan, P., Kumar, Rajiv Ranjan, Sharma, B.D., Mendiratta, Sanjod Kumar, Sharma, D.
Format: Article
Language:English
Subjects:
Accuracy
amelogenin gene
Blindness
Cattle
cattle meat
Chromosomes
Deoxyribonucleic acid
DNA
duplex PCR
Genes
Meat
Polymerase chain reaction
Primers
Sex
Sex determination
Slaughter
SRY gene
SRY protein
Y chromosome
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Summary:Analysis of the sex of cattle meat by fast and reliable molecular techniques is an important measure to ensure correct allocation of export refunds, particularly in European countries and also in countries such as India where female cattle (cow) slaughter is banned legally in many areas because of religious beliefs. Based on the male-specific SRY gene, X and Y chromosome specific amelogenin gene 2 pair of primers was designed and the system of PCR was optimized. Upon PCR amplification, male tissue showed 3 bands, while female tissue resulted in only 1 band. The accuracy and specificity of the primers were assessed using DNA template extracted from cattle meat of known sex. The protocol was subjected to a blind test and showed 100% concordance, proving its accuracy and reliability.
ISSN:0890-5436
1532-4249
DOI:10.1080/08905436.2011.645940