Deficiency of autoimmune regulator impairs the immune tolerance effect of bone marrow-derived dendritic cells in mice

• Published in: Autoimmunity (Chur, Switzerland) Switzerland), 2018-01, Vol.51 (1), p.10-17
• Main Authors: Huo, Feifei, Li, Dongbei, Zhao, Bo, Luo, Yadong, Zhao, Bingjie, Zou, Xueyang, Li, Yi, Yang, Wei
• Format: Article
• Language: English
• Subjects: AIRE Protein; Animals; Autoimmune regulator; Bone Marrow Cells - immunology; Bone Marrow Cells - pathology; CD4; cd4+ t cell; dendritic cells; Dendritic Cells - immunology; Dendritic Cells - pathology; immune tolerance; Mice; Mice, Knockout; Polyendocrinopathies, Autoimmune - immunology; Polyendocrinopathies, Autoimmune - pathology; T cell; T-Lymphocytes, Helper-Inducer - immunology; T-Lymphocytes, Helper-Inducer - pathology; tissue-restricted antigens; Transcription Factors - genetics; Transcription Factors - immunology
• ISSN: 0891-6934; 1607-842X
• DOI: 10.1080/08916934.2017.1422124

As a transcription factor, autoimmune regulator (Aire) participates in thymic negative selection and maintains immune tolerance mainly by regulating the ectopic expression of tissue-restricted antigens (TRAs) in medullary thymic epithelial cells (mTECs). Aire is also expressed in dendritic cells (DCs). DCs are professional antigen-presenting cells (APCs) that affect the differentiation of T cells toward distinct subpopulations and participate in the immune response and tolerance, thereby playing an important role in maintaining homeostasis. To determine the role of Aire in maintaining immune tolerance by bone marrow-derived dendritic cells (BMDCs), in the present study we utilized Aire-knockout mice to examine the changes of maturation status and TRAs expression on BMDCs, additionally investigate the differentiation of CD4 + T cells. The results showed that expression of costimulatory molecule and major histocompatibility complex class II (MHC-II) molecule was increased and expression of various TRAs was decreased in BMDCs from Aire-knockout mice. Aire deficiency reduced the differentiation of naïve CD4 + T cells into type 2T helper (Th2) cells and regulatory T cells (Tregs) but enhanced the differentiation of naïve CD4 + T cells into Th1 cells, Th17 cells, and follicular helper T (Tfh) cells. The results demonstrate that Aire expressed by BMDCs plays an important role in the maintenance of homeostasis by regulating TRA expression and the differentiation of T cell subsets.