Inhibition of the NF-κB and p38 MAPK pathways by scopoletin reduce the inflammation caused by carrageenan in the mouse model of pleurisy

Natural products have long been used worldwide as therapeutic agents, but it is only recently, in response to the new challenges posed by global population aging, that interest in research into potentially therapeutic natural products has reemerged. In this context, coumarins, chemical compounds fou...

Full description

Saved in:
Bibliographic Details
Published in:Immunopharmacology and immunotoxicology 2016-09, Vol.38 (5), p.344-352
Main Authors: Pereira dos Santos Nascimento, Marcus Vinicius, Arruda-Silva, Fábio, Gobbo Luz, Ana Beatriz, Baratto, Bruna, Venzke, Dalila, Mendes, Beatriz Garcia, Fröde, Tânia Silvia, Geraldo Pizzolatti, Moacir, Dalmarco, Eduardo Monguilhott
Format: Article
Language:English
Subjects:
Airway inflammation
Animals
anti-inflammatory
Carrageenan - toxicity
Disease Models, Animal
Female
Inflammation - chemically induced
Inflammation - drug therapy
Inflammation - immunology
MAP Kinase Signaling System - drug effects
Mice
NF-kappa B - immunology
p38 Mitogen-Activated Protein Kinases - immunology
pleurisy
Pleurisy - chemically induced
Pleurisy - drug therapy
Pleurisy - immunology
scopoletin
Scopoletin - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Natural products have long been used worldwide as therapeutic agents, but it is only recently, in response to the new challenges posed by global population aging, that interest in research into potentially therapeutic natural products has reemerged. In this context, coumarins, chemical compounds found in plants that have known anti-inflammatory activity, are promising candidates for the development of new drugs. In this study we test the effect of scopoletin, a coumarin found in several plant species, on carrageenan-induced inflammation in the mouse model of pleurisy. Initially, the effects of scopoletin on leukocyte migration and exudate concentrations were evaluated at three different doses (0.1, 1 and 5 mg/kg) and time (0.5-4 h before pleurisy). In the next step, we chose the lowest dose capable of inhibiting the inflammatory parameters (1 mg/kg), in order to analyze the myeloperoxidase and adenosine deaminase activities, the nitric oxide, tumor necrosis factor-α, and interleukin 1β levels in the fluid leakage, and the p65 subunit of NF-κB and p38 MAPK phosphorylation. Scopoletin at a dose of 1 mg/kg was able to significantly reduce cell migration and exudation to the pleural fluid (p 
ISSN:0892-3973
1532-2513
DOI:10.1080/08923973.2016.1203929