Lactoferrin and ovotransferrin contribute toward antioxidative effects of Edible Bird's Nest against hydrogen peroxide-induced oxidative stress in human SH-SY5Y cells

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2015-10, Vol.79 (10), p.1570-1578
• Main Authors: Hou, Zhiping, Imam, Mustapha Umar, Ismail, Maznah, Azmi, Nur Hanisah, Ismail, Norsharina, Ideris, Aini, Mahmud, Rozi
• Format: Article
• Language: English
• Subjects: Animals; antioxidant; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological Products - chemistry; Birds; Cell Line, Tumor; Conalbumin - isolation & purification; Conalbumin - pharmacology; Edible Bird's Nest; Gene Expression - drug effects; Humans; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - pharmacology; lactoferrin; Lactoferrin - isolation & purification; Lactoferrin - pharmacology; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; neuroprotection; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; ovotransferrin; Oxidative Stress - drug effects; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases - genetics; Poly(ADP-ribose) Polymerases - metabolism; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase-1
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2015.1050989

There are reports of improved redox outcomes due to consumption of Edible Bird's Nest (EBN). Many of the functional effects of EBN can be linked to its high amounts of antioxidants. Interestingly, dietary components with high antioxidants have shown promise in the prevention of aging and its related diseases like Alzheimer's disease. In this study, the antioxidative potentials of EBN and its constituents, lactoferrin (LF) and ovotransferrin (OVF), were determined and protective effects against hydrogen peroxide (H 2 O 2 )- induced toxicity on SH-SY5Y cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and acridine orange and propidium iodide (AO/PI) staining with microscopy were examined. Results showed that EBN and its constituents attenuated H 2 O 2 -induced cytotoxicity, and decreased radical oxygen species (ROS) through increased scavenging activity. Furthermore, LF, OVF, and EBN produced transcriptional changes in antioxidant related genes that tended towards neuroprotection as compared to H 2 O 2 -treated group. Overall, the results suggest that LF and OVF may produce synergistic or all-or-none antioxidative effects in EBN. The Edible Bird's Nest-induced neuroprotection activity was possibly related to its ability of antioxidant system as well as through activation of SOD1/SOD2/PARP1 transcriptional genes