Characterization of a new Lactobacillus salivarius strain engineered to express IBV multi-epitope antigens by chromosomal integration

To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-03, Vol.80 (3), p.574-583
Main Authors: Ma, Bing-cun, Yang, Xin, Wang, Hong-ning, Cao, Hai-peng, Xu, Peng-wei, Ding, Meng-die, Liu, Hui
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - genetics
Chickens
chromosomal integration
Chromosomes, Bacterial
Epitopes - genetics
Female
Lactobacillus - genetics
Lactobacillus - immunology
Lactobacillus salivarius
Male
thyA-deficient
Tibetan chicken
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on chromosomal integration strategy. We demonstrated that a new Lactobacillu salivarius TCMM17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is susceptible to tested antimicrobials, and shows no toxicities. In order to examine the potential of TCMM17 strain as heterogenous antigen delivering vehicle, we introduced a UTEpi C-A expression cassette in its chromosome by constructing a non-replicative plasmid (pORI280-UUTEpi C-AD). The recombinant TCMM17 strain (∆TCMM17) stably was found to keep the gene cassette through 50 generations, and successfully displayed EpiC encoded by the cassette on its surface. This work provides a universal platform for development of novel oral vaccines and expression of further antigens of avian pathogens. Schematic representation of the non-replicative integration plasmid pORI280-UUTEpi C-AD construction and the overview of the homologous recombination strategy.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2015.1101330