3,4-Dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2017-10, Vol.81 (10), p.1978-1983
• Main Authors: Liu, Yujia, Kurita, Ayuki, Nakashima, Sayaka, Zhu, Beiwei, Munemasa, Shintaro, Nakamura, Toshiyuki, Murata, Yoshiyuki, Nakamura, Yoshimasa
• Format: Article
• Language: English
• Subjects: 3,4-dihydroxyphenylacetic acid; 3,4-Dihydroxyphenylacetic Acid - pharmacology; acetaldehyde; Acetaldehyde - toxicity; aldehyde dehydrogenase; Aldehyde Dehydrogenase - biosynthesis; Aldehyde Dehydrogenase - genetics; Aldehyde Dehydrogenase - metabolism; Animals; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Dose-Response Relationship, Drug; Enzyme Induction - drug effects; Gene Expression Regulation, Enzymologic - drug effects; Hepa1c1c7 cells; Liver Neoplasms - pathology; Mice; Nrf2
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2017.1361809

3,4-Dihydroxyphenylacetic acid (DOPAC) is one of the major colonic microflora-produced catabolites of quercetin glycosides, such as quercetin 4′-glucoside derived from onion. Here, we investigated whether DOPAC modulates the aldehyde dehydrogenase (ALDH) activity and protects the cells from the acetaldehyde-induced cytotoxicity in vitro. DOPAC was shown to enhance not only the total ALDH activity, but also the gene expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. DOPAC simultaneously stimulated the nuclear translocation of NFE2-related factor 2 and aryl hydrocarbon receptor. The pretreatment of DOPAC completely protected the cells from the acetaldehyde-induced cytotoxicity. The present study suggested that DOPAC acts as a potential ALDH inducer to prevent the alcohol-induced abnormal reaction. 3,4-Dihydroxyphenylacetic acid, a colonic microflora-produced catabolite of quercetin glycosides, protects the cells from the acetaldehyde-induced cytotoxicity.