Synthesis, characterization, and biological activity of cross-linked chitosan biguanidine loaded with silver nanoparticles

Chitosan biguanidine hydrochloride (ChG) and glutaraldehyde cross-linked chitosan biguanidine (CChG) were synthesized and characterized by Fourier transform infrared spectroscopy, 1 H NMR and 13 C NMR, X-ray diffraction, scanning electron microscopy (SEM) and thermal analyses (TGA and DTA). The resu...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2016-12, Vol.27 (18), p.1880-1898
Main Authors: Salama, Hend E., Saad, Gamal R., Sabaa, Magdy W.
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
antimicrobial activity
Aspergillus fumigatus
Bacillus subtilis
Biocompatibility
Chitosan
Chitosan biguanidine
cross-linked
Crosslinking
cytotoxicity
Escherichia coli
Nanoparticles
Scanning electron microscopy
Silver
silver nanoparticles
Streptococcus pneumoniae
Synthesis
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Summary:Chitosan biguanidine hydrochloride (ChG) and glutaraldehyde cross-linked chitosan biguanidine (CChG) were synthesized and characterized by Fourier transform infrared spectroscopy, 1 H NMR and 13 C NMR, X-ray diffraction, scanning electron microscopy (SEM) and thermal analyses (TGA and DTA). The results showed that ChG and CChG had a more amorphous structure than that of chitosan, and their thermal stability were slightly lower than that of chitosan. Colloidal silver nanoparticles (AgNPs) were prepared using borohydride reduction method and then investigated as fillers in partially cross-linked chitosan biguanidine. The obtained nanoparticles were uniform and spherical with average size of 9.6 ± 0.5 nm. The prepared CChG/AgNPs composites were characterized for their morphology, thermal properties, cytotoxicity and antimicrobial activity. The SEM images showed that the AgNPs are well imbedded in the CChG matrix. The thermal stability of CChG was improved with incorporation of AgNPs. The CChG and CChG/AgNPs showed less cytotoxicity to breast cancer cells (MCF-7). Compared with chitosan and CChG, the ChG and CChG/AgNPs showed better antimicrobial activity against Streptococcus pneumoniae and Bacillus subtilis as Gram-positive bacteria, Escherichia coli as Gram-negative bacteria and Aspergillus fumigatus, Geotricum candidum and Syncephalastrum recemosum as fungi.
ISSN:0920-5063
1568-5624
DOI:10.1080/09205063.2016.1239950