Evaluation of Potential Antigen-specific Host Biomarkers in QuantiFERON Supernatants as Candidates for the Diagnosis of Ocular Tuberculosis

Purpose: To evaluate potential host biomarkers detectable in QuantiFERON supernatants as diagnostic candidates for ocular tuberculosis (OTB). Methods: We investigated 47 host markers in QuantiFERON supernatants from 92 individuals with uveitis using the Luminex platform. We evaluated the potential o...

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Bibliographic Details
Published in:Ocular immunology and inflammation 2021-11, Vol.29 (7-8), p.1480-1488
Main Authors: Makhoba, Nonjabulo S., Smit, Derrick P., Walzl, Gerhard, Chegou, Novel N.
Format: Article
Language:English
Subjects:
Adult
Antigens, Bacterial - immunology
Biomarkers - blood
candidate biosignatures
CD40 Ligand - blood
diagnosis
Female
HIV Infections - complications
Host biomarkers
Humans
Interferon-gamma - blood
Interferon-gamma Release Tests
Interleukin-33 - blood
Male
Middle Aged
Mycobacterium tuberculosis - immunology
ocular tuberculosis
quantiferon supernatants
ROC Curve
Sensitivity and Specificity
Serum Amyloid P-Component - metabolism
Tuberculosis, Ocular - blood
Tuberculosis, Ocular - diagnosis
Uveitis - blood
Uveitis - diagnosis
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Summary:Purpose: To evaluate potential host biomarkers detectable in QuantiFERON supernatants as diagnostic candidates for ocular tuberculosis (OTB). Methods: We investigated 47 host markers in QuantiFERON supernatants from 92 individuals with uveitis using the Luminex platform. We evaluated the potential of individual and combined biomarkers to distinguish between patients with possible, probable, and no OTB. Results: Differences were observed in median concentrations of several biomarkers including IL-13, IFN-γ, IFN-α2, and IL-1β, in individuals with OTB versus no OTB regardless of HIV status. Individuals with probable and possible OTB only differed regarding GM-CSF. We identified a four-marker biosignature (CD40 L, IL-33, IFN-γ, and SAP) which diagnosed OTB with an area under the ROC curve of 0.80, sensitivity = 56.3% and specificity = 90.0%. Conclusion: This represents the first attempt at screening QuantiFERON supernatants for biomarkers to diagnose OTB. We identified candidate biosignatures which may aid in diagnosing OTB in both HIV positive and negative patients.
ISSN:0927-3948
1744-5078
DOI:10.1080/09273948.2020.1763404