Sorting of micron-sized particles using holographic optical Raman tweezers in aqueous medium

We have constructed a holographic optical tweezers system combined with Raman spectroscopy to sort trapped particles. Our software automatically moves the trapped objects to the measurement positions to obtain individual Raman signals from multiple trapped particles. We performed the sorting by comp...

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Bibliographic Details
Published in:Journal of modern optics 2019-01, Vol.66 (2), p.228-234
Main Authors: Parlatan, Uğur, Başar, Gönül, Başar, Günay
Format: Article
Language:English
Subjects:
Aqueous solutions
Beads
Correlation analysis
Correlation coefficients
Holographic optical tweezers
multivariate statistical analysis
Object motion
particle sorting
Polystyrene resins
Raman spectroscopy
Spectroscopy
Spectrum analysis
Trapped particles
Yeast
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Summary:We have constructed a holographic optical tweezers system combined with Raman spectroscopy to sort trapped particles. Our software automatically moves the trapped objects to the measurement positions to obtain individual Raman signals from multiple trapped particles. We performed the sorting by comparing their spectra with the previously measured training dataset using the correlation coefficients. We used yeast cells and polystyrene beads as test particles. This study aims to show that biological particles can be separated using single cell analysis with combined holographic optical tweezers and Raman spectroscopy system.
ISSN:0950-0340
1362-3044
DOI:10.1080/09500340.2018.1515378