Oxytocin receptor- and Oct-4-expressing cells in human amniotic fluid

Background aims. The present clinical and molecular study aimed at investigating the presence of the genes encoding oxytocin receptor (OT-R) and Oct-4 in human amniotic fluid cells. Methods. Amniotic fluid samples were obtained from amniocentesis. Cells from human amniotic fluid samples were analyze...

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Published in:Gynecological endocrinology 2008-05, Vol.24 (5), p.280-284
Main Authors: Stefanidis, Konstantinos, Loutradis, Dimitris, Anastasiadou, Vasiliki, Bletsa, Ritsa, Kiapekou, Erasmia, Drakakis, Peter, Beretsos, Panos, Elenis, Evangelia, Mesogitis, Spiros, Antsaklis, Aris
Format: Article
Language:English
Subjects:
Adult
Amniotic Fluid - cytology
Amniotic Fluid - metabolism
Amniotic fluid cells
Female
Humans
Immunohistochemistry
Oct-4 gene
Octamer Transcription Factor-3 - biosynthesis
Octamer Transcription Factor-3 - genetics
Oxytocin - biosynthesis
Oxytocin - genetics
oxytocin receptor gene
polymerase chain reaction
Pregnancy
Receptors, Oxytocin - biosynthesis
Receptors, Oxytocin - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
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Summary:Background aims. The present clinical and molecular study aimed at investigating the presence of the genes encoding oxytocin receptor (OT-R) and Oct-4 in human amniotic fluid cells. Methods. Amniotic fluid samples were obtained from amniocentesis. Cells from human amniotic fluid samples were analyzed for mRNA expression of OT-R and Oct-4 via reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemistry was also performed with OT-R and Oct-4 antibodies. Results. RT-PCR from 10 independent amniocentesis samples demonstrated the expression of OT-R and Oct-4 mRNA. The cells also showed strong immunoreactivity for molecular markers of OT-R and Oct-4. Conclusion. OT-R and Oct-4 are expressed in human amniotic fluid cells. The role of oxytocin in the physiology and pathophysiology of amniotic fluid cells remains to be settled.
ISSN:0951-3590
1473-0766
DOI:10.1080/09513590801977167