Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay

Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-re...

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Bibliographic Details
Published in:Food and agricultural immunology 2018-01, Vol.29 (1), p.711-721
Main Authors: Zhang, Yong, Peng, Juan, Guo, Ping, Li, Guo-Qiang, Zhang, Kai-Yi, Lv, Xi, Lai, Wei-Hua
Format: Article
Language:English
Subjects:
Clenbuterol
Cost analysis
Fluorescence
Food safety
Gold
immunochromatographic assay
Livestock
Matrix effects
Sensitivity analysis
Swine
Urine
Working conditions
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Summary:Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-resolved fluorescent nanobead ICA (TRFN-ICA) and colloidal gold ICA (CG-ICA) were developed to detect clenbuterol in swine urine. Under optimized working conditions, the limits of detection of TRFN-ICA and CG-ICA were 16 and 68 pg/mL, respectively. The matrix effect on TRFN-ICA and CG-ICA was assessed in 20 swine urine samples. Results indicated that the sensitivity of TRFN-ICA was better than that of CG-ICA, and the detection time of the former was shorter than that of the latter. The matrix effect on TRFN-ICA was more serious than that on CG-ICA.
ISSN:0954-0105
1465-3443
DOI:10.1080/09540105.2018.1439456