A fluorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

• Published in: Food and agricultural immunology 2019-01, Vol.30 (1), p.208-221
• Main Authors: Xie, Yong, Wu, Juan, Shi, Haixing, Le, Tao
• Format: Article
• Language: English
• Subjects: 3-amino-5-methylmorpholino-2-oxazolidinone; animal tissue; Animal tissues; Cell lines; Coefficient of variation; Fluorescence; Furaltadone; immunochromatographic strip; Metabolites; Monoclonal antibodies; quantum dot; Quantum dots; Strip
• ISSN: 0954-0105; 1465-3443
• DOI: 10.1080/09540105.2019.1566301

To monitor the levels of furaltadone in animal tissues, an immunochromatographic strip (immunostrip) based on quantum dot was developed for rapid detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), the metabolite of furaltadone. Four monoclonal antibodies (mAbs) against nitrophenyl derivative of AMOZ (2-NP-AMOZ) were generated from the hybridoma cell lines. Among them, the mAb 5C10/3A8 showed the highest sensitivity to 2-NP-AMOZ. Using an immunostrip reader, with the 2-NP-AMOZ as the target, the immunostrip had a liner range from 1 to 30 ng/mL, with 50% inhibition concentration of 5.22 ng/mL and limit of detection of 0.07 ng/mL. The recoveries for AMOZ ranged from 78.6% to 108.9%, with coefficients of variation below 12%. A good correlation (R 2  = 0.9901) between the immunostrip and LC-MS/MS results in the tissues tested demonstrated the reliability of the immunostrip. These results indicate that proposed immunostrip is suitable for the rapid and quantitative detection of AMOZ residues in edible animal tissues.