Loading…
Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study
Purpose: This study was designed to investigate the applicability of carbomer 940 hydrogel on skin-burn wounds by focusing on its effects on the zone of stasis. Method: Fibroblast cells were used to assess the cytotoxicity of carbomer 940. Wound healing was monitored by laser Doppler flowmetry and h...
Saved in:
| Published in: | The Journal of dermatological treatment 2018-08, Vol.29 (6), p.593-599 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73 |
| container_end_page | 599 |
| container_issue | 6 |
| container_start_page | 593 |
| container_title | The Journal of dermatological treatment |
| container_volume | 29 |
| creator | Hayati, Farzad Ghamsari, Seyed Mehdi Dehghan, Mohammad Mehdi Oryan, Ahmad |
| description | Purpose: This study was designed to investigate the applicability of carbomer 940 hydrogel on skin-burn wounds by focusing on its effects on the zone of stasis.
Method: Fibroblast cells were used to assess the cytotoxicity of carbomer 940. Wound healing was monitored by laser Doppler flowmetry and histopathological analysis. Twenty adult male Wistar rats were randomly divided into two groups (N = 10 each). After shaving their dorsum, a 'burn comb' was contacted bilaterally on each side of the spine. Carbomer 940 hydrogel and TegaDerm were used as topical dressing, in the experimental group, while normal saline and TegaDerm were used as the control. The dressing was changed daily and the blood flow was measured until the animals were euthanized. The wound samples were collected at days 3 and 21 for histopathological studies. Cell viability study showed that the hydrogel was non-cytotoxic.
Result: Our results revealed that skin blood perfusion in the zone of stasis was elevated at 24 hour post-burning, in the experimental group but not in the control group (p |
| doi_str_mv | 10.1080/09546634.2018.1426823 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_09546634_2018_1426823</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1989570056</sourcerecordid><originalsourceid>FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EglL4BJCXbFLsjO3ErEBVeUgVbGBtOX5AUBKDnYDy96RqYclqHjozVzoInVGyoKQkl0RyJgSwRU5ouaAsF2UOe2hGWUEzWnDYR7MNk22gI3Sc0jshFAQpD9FRLoFOvJyhx5X3zvQJB4-NjlVoXcSSEfw22hheXYNDh6shdvg7DJ1NV1h3uO7wV93HMPV2O3wFnPrBjifowOsmudNdnaOX29Xz8j5bP909LG_WmQEh-kwzS43gANx66TmworISNHEMKgAopa0KQ4qClXlJwBsuaW4IuGpaeeELmKOL7d-PGD4Hl3rV1sm4ptGdC0NSVJaSF4RwMaF8i5oYUorOq49YtzqOihK1Ual-VaqNSrVTOd2d7yKGqnX27-rX3QRcb4G68yG2-jvExqpej02IPurO1EnB_xk_a5WA6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1989570056</pqid></control><display><type>article</type><title>Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study</title><source>Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list)</source><creator>Hayati, Farzad ; Ghamsari, Seyed Mehdi ; Dehghan, Mohammad Mehdi ; Oryan, Ahmad</creator><creatorcontrib>Hayati, Farzad ; Ghamsari, Seyed Mehdi ; Dehghan, Mohammad Mehdi ; Oryan, Ahmad</creatorcontrib><description>Purpose: This study was designed to investigate the applicability of carbomer 940 hydrogel on skin-burn wounds by focusing on its effects on the zone of stasis.
Method: Fibroblast cells were used to assess the cytotoxicity of carbomer 940. Wound healing was monitored by laser Doppler flowmetry and histopathological analysis. Twenty adult male Wistar rats were randomly divided into two groups (N = 10 each). After shaving their dorsum, a 'burn comb' was contacted bilaterally on each side of the spine. Carbomer 940 hydrogel and TegaDerm were used as topical dressing, in the experimental group, while normal saline and TegaDerm were used as the control. The dressing was changed daily and the blood flow was measured until the animals were euthanized. The wound samples were collected at days 3 and 21 for histopathological studies. Cell viability study showed that the hydrogel was non-cytotoxic.
Result: Our results revealed that skin blood perfusion in the zone of stasis was elevated at 24 hour post-burning, in the experimental group but not in the control group (p < .05) and this trend continued until the animals were euthanized. Histopathologically, there was statistically more epithelial tissue in the zone of stasis in the experimental group than in the control group, and the sebaceous glands and hair follicles remained viable in the group treated by hydrogel on day 3. There was significantly more collagen deposition and fibroplasia in the ischemic zones of the experimental group than in the control group at day 21.
Conclusion: Our study has shown that carbomer 940 hydrogel is a nontoxic biomaterial, which improved tissue perfusion and decreased the area of necrotic tissue in burn wounds. Application of this biomaterial in treatment of burn injuries deserves further studies.</description><identifier>ISSN: 0954-6634</identifier><identifier>EISSN: 1471-1753</identifier><identifier>DOI: 10.1080/09546634.2018.1426823</identifier><identifier>PMID: 29316829</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Acrylic Resins - chemistry ; Acrylic Resins - pharmacology ; Acrylic Resins - therapeutic use ; Administration, Topical ; Animals ; burn-wound healing ; Burns - drug therapy ; Carbomer 940 ; Cell Survival - drug effects ; cytotoxicity ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Laser-Doppler Flowmetry ; Male ; rat ; Rats ; Rats, Wistar ; Skin - drug effects ; Skin - pathology ; Wound Healing - drug effects ; zone of stasis</subject><ispartof>The Journal of dermatological treatment, 2018-08, Vol.29 (6), p.593-599</ispartof><rights>2018 Informa UK Limited, trading as Taylor & Francis Group 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73</citedby><cites>FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29316829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hayati, Farzad</creatorcontrib><creatorcontrib>Ghamsari, Seyed Mehdi</creatorcontrib><creatorcontrib>Dehghan, Mohammad Mehdi</creatorcontrib><creatorcontrib>Oryan, Ahmad</creatorcontrib><title>Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study</title><title>The Journal of dermatological treatment</title><addtitle>J Dermatolog Treat</addtitle><description>Purpose: This study was designed to investigate the applicability of carbomer 940 hydrogel on skin-burn wounds by focusing on its effects on the zone of stasis.
Method: Fibroblast cells were used to assess the cytotoxicity of carbomer 940. Wound healing was monitored by laser Doppler flowmetry and histopathological analysis. Twenty adult male Wistar rats were randomly divided into two groups (N = 10 each). After shaving their dorsum, a 'burn comb' was contacted bilaterally on each side of the spine. Carbomer 940 hydrogel and TegaDerm were used as topical dressing, in the experimental group, while normal saline and TegaDerm were used as the control. The dressing was changed daily and the blood flow was measured until the animals were euthanized. The wound samples were collected at days 3 and 21 for histopathological studies. Cell viability study showed that the hydrogel was non-cytotoxic.
Result: Our results revealed that skin blood perfusion in the zone of stasis was elevated at 24 hour post-burning, in the experimental group but not in the control group (p < .05) and this trend continued until the animals were euthanized. Histopathologically, there was statistically more epithelial tissue in the zone of stasis in the experimental group than in the control group, and the sebaceous glands and hair follicles remained viable in the group treated by hydrogel on day 3. There was significantly more collagen deposition and fibroplasia in the ischemic zones of the experimental group than in the control group at day 21.
Conclusion: Our study has shown that carbomer 940 hydrogel is a nontoxic biomaterial, which improved tissue perfusion and decreased the area of necrotic tissue in burn wounds. Application of this biomaterial in treatment of burn injuries deserves further studies.</description><subject>Acrylic Resins - chemistry</subject><subject>Acrylic Resins - pharmacology</subject><subject>Acrylic Resins - therapeutic use</subject><subject>Administration, Topical</subject><subject>Animals</subject><subject>burn-wound healing</subject><subject>Burns - drug therapy</subject><subject>Carbomer 940</subject><subject>Cell Survival - drug effects</subject><subject>cytotoxicity</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Laser-Doppler Flowmetry</subject><subject>Male</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Skin - drug effects</subject><subject>Skin - pathology</subject><subject>Wound Healing - drug effects</subject><subject>zone of stasis</subject><issn>0954-6634</issn><issn>1471-1753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EglL4BJCXbFLsjO3ErEBVeUgVbGBtOX5AUBKDnYDy96RqYclqHjozVzoInVGyoKQkl0RyJgSwRU5ouaAsF2UOe2hGWUEzWnDYR7MNk22gI3Sc0jshFAQpD9FRLoFOvJyhx5X3zvQJB4-NjlVoXcSSEfw22hheXYNDh6shdvg7DJ1NV1h3uO7wV93HMPV2O3wFnPrBjifowOsmudNdnaOX29Xz8j5bP909LG_WmQEh-kwzS43gANx66TmworISNHEMKgAopa0KQ4qClXlJwBsuaW4IuGpaeeELmKOL7d-PGD4Hl3rV1sm4ptGdC0NSVJaSF4RwMaF8i5oYUorOq49YtzqOihK1Ual-VaqNSrVTOd2d7yKGqnX27-rX3QRcb4G68yG2-jvExqpej02IPurO1EnB_xk_a5WA6A</recordid><startdate>20180818</startdate><enddate>20180818</enddate><creator>Hayati, Farzad</creator><creator>Ghamsari, Seyed Mehdi</creator><creator>Dehghan, Mohammad Mehdi</creator><creator>Oryan, Ahmad</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180818</creationdate><title>Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study</title><author>Hayati, Farzad ; Ghamsari, Seyed Mehdi ; Dehghan, Mohammad Mehdi ; Oryan, Ahmad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Acrylic Resins - pharmacology</topic><topic>Acrylic Resins - therapeutic use</topic><topic>Administration, Topical</topic><topic>Animals</topic><topic>burn-wound healing</topic><topic>Burns - drug therapy</topic><topic>Carbomer 940</topic><topic>Cell Survival - drug effects</topic><topic>cytotoxicity</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogels - pharmacology</topic><topic>Laser-Doppler Flowmetry</topic><topic>Male</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Skin - drug effects</topic><topic>Skin - pathology</topic><topic>Wound Healing - drug effects</topic><topic>zone of stasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hayati, Farzad</creatorcontrib><creatorcontrib>Ghamsari, Seyed Mehdi</creatorcontrib><creatorcontrib>Dehghan, Mohammad Mehdi</creatorcontrib><creatorcontrib>Oryan, Ahmad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of dermatological treatment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hayati, Farzad</au><au>Ghamsari, Seyed Mehdi</au><au>Dehghan, Mohammad Mehdi</au><au>Oryan, Ahmad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study</atitle><jtitle>The Journal of dermatological treatment</jtitle><addtitle>J Dermatolog Treat</addtitle><date>2018-08-18</date><risdate>2018</risdate><volume>29</volume><issue>6</issue><spage>593</spage><epage>599</epage><pages>593-599</pages><issn>0954-6634</issn><eissn>1471-1753</eissn><abstract>Purpose: This study was designed to investigate the applicability of carbomer 940 hydrogel on skin-burn wounds by focusing on its effects on the zone of stasis.
Method: Fibroblast cells were used to assess the cytotoxicity of carbomer 940. Wound healing was monitored by laser Doppler flowmetry and histopathological analysis. Twenty adult male Wistar rats were randomly divided into two groups (N = 10 each). After shaving their dorsum, a 'burn comb' was contacted bilaterally on each side of the spine. Carbomer 940 hydrogel and TegaDerm were used as topical dressing, in the experimental group, while normal saline and TegaDerm were used as the control. The dressing was changed daily and the blood flow was measured until the animals were euthanized. The wound samples were collected at days 3 and 21 for histopathological studies. Cell viability study showed that the hydrogel was non-cytotoxic.
Result: Our results revealed that skin blood perfusion in the zone of stasis was elevated at 24 hour post-burning, in the experimental group but not in the control group (p < .05) and this trend continued until the animals were euthanized. Histopathologically, there was statistically more epithelial tissue in the zone of stasis in the experimental group than in the control group, and the sebaceous glands and hair follicles remained viable in the group treated by hydrogel on day 3. There was significantly more collagen deposition and fibroplasia in the ischemic zones of the experimental group than in the control group at day 21.
Conclusion: Our study has shown that carbomer 940 hydrogel is a nontoxic biomaterial, which improved tissue perfusion and decreased the area of necrotic tissue in burn wounds. Application of this biomaterial in treatment of burn injuries deserves further studies.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>29316829</pmid><doi>10.1080/09546634.2018.1426823</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0954-6634 |
| ispartof | The Journal of dermatological treatment, 2018-08, Vol.29 (6), p.593-599 |
| issn | 0954-6634 1471-1753 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_09546634_2018_1426823 |
| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Medical Collection (Reading list) |
| subjects | Acrylic Resins - chemistry Acrylic Resins - pharmacology Acrylic Resins - therapeutic use Administration, Topical Animals burn-wound healing Burns - drug therapy Carbomer 940 Cell Survival - drug effects cytotoxicity Hydrogels - chemistry Hydrogels - pharmacology Laser-Doppler Flowmetry Male rat Rats Rats, Wistar Skin - drug effects Skin - pathology Wound Healing - drug effects zone of stasis |
| title | Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A46%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20carbomer%20940%20hydrogel%20on%20burn%20wounds:%20an%20in%20vitro%20and%20in%20vivo%20study&rft.jtitle=The%20Journal%20of%20dermatological%20treatment&rft.au=Hayati,%20Farzad&rft.date=2018-08-18&rft.volume=29&rft.issue=6&rft.spage=593&rft.epage=599&rft.pages=593-599&rft.issn=0954-6634&rft.eissn=1471-1753&rft_id=info:doi/10.1080/09546634.2018.1426823&rft_dat=%3Cproquest_cross%3E1989570056%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c366t-a4d1c65335df9f5347bd93a0e43b33389db7c077482803fc5912c03eb774f6f73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1989570056&rft_id=info:pmid/29316829&rfr_iscdi=true |