Quantitative proteomic analysis of the effects of melatonin treatment for mice suffered from small intestinal damage induced by γ-ray radiation

• Published in: International journal of radiation biology 2021-09, Vol.97 (9), p.1206-1216
• Main Authors: Wang, Qin, Wang, Yan, Du, Liqing, Xu, Chang, Liu, Yang, Liu, Qiang, Fan, Saijun
• Format: Article
• Language: English
• Subjects: abdominal irradiation; Animals; Gamma Rays - adverse effects; intestinal damage; Intestine, Small - drug effects; Intestine, Small - metabolism; Intestine, Small - pathology; Intestine, Small - radiation effects; Male; Melatonin; Melatonin - pharmacology; Mice; Mice, Inbred C57BL; Proteomics; Radiation Injuries, Experimental - drug therapy; Radiation Injuries, Experimental - metabolism; Radiation Injuries, Experimental - pathology; Radiation-Protective Agents - pharmacology; radioprotection
• ISSN: 0955-3002; 1362-3095; 1362-3095
• DOI: 10.1080/09553002.2021.1956006

Intestinal damage induced by radiation exposure is a major clinic concern of radiotherapy for patients with abdominal or pelvic tumor. Melatonin (N-acetyl-5-methoxytryptamine) is likely be an ideal radioprotector to protect individuals from radiation exposure. The study aimed to define the role of melatonin in small intestinal damage caused by abdominal irradiation (ABI). 30-day survival rate and pathological histology of the intestines from melatonin-treated mice after 13 Gy ABI exposure was first detected. Next, quantitative proteomics analysis of the small intestines tissue was examined and GO term and KEGG pathways analysis were performed. Melatonin treatment before ABI exposure significantly increased 30-day survival rate to 83% and ameliorated damage to the intestinal epithelial cells. Melatonin significantly altered the proteins profile of the small intestines following irradiation. For the irradiated mice treated with melatonin in comparison with the irradiated mice, the enriched GO terms were mainly involved in defense response to other organism (BP, GO: 0098542), response to other organism (BP, GO: 0051707), anion transmembrane transporter activity (MF, GO: 0008509), and secondary active transmembrane transporter activity (MF, GO: 0015291). In the process of antioxidant activity (MF, GO: 0016209), melatonin treatment prior to radiation exhibited high protein levels of Sod3 and Gpx3. The markedly KEGG pathways for melatonin treatment prior to radiation mainly included protein digestion and absorption (ko 04974) and mineral absorption (ko 04978). p53 signaling pathway and DNA repair pathways were enriched in melatonin treated mice. The amount of radiation-induced DNA damage and the cell apoptosis of the small intestines was decreased in the melatonin-treated mice. Melatonin may protect small intestines from radiation damage through increasing DNA repair and decreasing cell apoptosis of the small intestines. Our data provided perspective for the study of melatonin in mitigating ABI-caused intestinal damage.