L. reuteri JMR-01 adjuvant 12 C 6+ irradiation exerts anti-colon carcinoma effects by modulating the gut microbiota in mice

Probiotics such as could modulate the intestinal microbiota and have been considered as an effective strategy for ameliorating colon carcinoma. Nevertheless, its efficiency remains the biggest challenge. We investigated the therapeutic efficacy of JMR-01 adjuvant C irradiation on CT-26 syngeneic mou...

Full description

Saved in:
Bibliographic Details
Published in:International journal of radiation biology 2023-05, Vol.99 (5), p.779-790
Main Authors: Bai, Jin, Wang, Shuyang, Xu, Fuqiang, Dong, Miaoyin, Wang, Junkai, Sun, Xisi, Xiao, Guoqing
Format: Article
Language:English
Subjects:
Animals
Carcinoma
Colonic Neoplasms - radiotherapy
Gastrointestinal Microbiome
Lactobacillus
Limosilactobacillus reuteri
Mice
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Probiotics such as could modulate the intestinal microbiota and have been considered as an effective strategy for ameliorating colon carcinoma. Nevertheless, its efficiency remains the biggest challenge. We investigated the therapeutic efficacy of JMR-01 adjuvant C irradiation on CT-26 syngeneic mouse models. Meanwhile, intestinal flora and innate immunity were examined to outline mechanisms. Anti-proliferation effect of live probiotic combined with inactivated probiotic JMR-01 (LP + IP) on CT-26 reached a maximum of 39.55% among other experiment groups at 24 h when the ratio of cell to CFU was 1:1 in vitro. These activities have been fully validated in vivo, tumor-bearing mice treated by C irradiation combining with living and inactivated probiotics JMR-01 (IR + LP + IP) for 50-day held the highest survival rate (71.4%) and complete remission rate (14.3%). We also demonstrated significant fluctuation in gut microbiota, including the decreased abundance of and related to tumorigenesis and development, and the increased abundance of and closely associated with health restoration in fecal of mice treated with JMR-01 LP + IP adjuvant C irradiation (IR + LP + IP). Similarly, the decreasing nitroreductase activities and increasing short chain fatty acids (SCFAs) concentrations were observed in IR + LP + IP group compared with tumor control group, which further confirmed the changes of gut microbiota. Additionally, we found that the strongest stimulation index of splenocyte (2.47) and the phagocytosis index peritoneal macrophage (3.68) were achieved by LP + IP compared with single live JMR-01 (LP) and inactivated JMR-01 (IP). JMR-01 LP + IP adjuvant C irradiation could mitigate cancer progression by modulating innate immunity as well as intestinal flora.
ISSN:0955-3002
1362-3095
DOI:10.1080/09553002.2023.2142979