Effect of postirradiation treatment on the radiation-induced haemolysis of human erythrocytes

The effect of postirradiation conditions on the haemolysis of gamma -irradiated (2.1 kGy) human erythrocytes was studied. Haemolysis was inhibited by incubation in mannitol and sucrose instead of saline, by hypertonicity of the medium and by calcium chelators. Dithiothreitol, butylated hydroxytoluen...

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Bibliographic Details
Published in:International journal of radiation biology 1997-03, Vol.71 (3), p.337-343
Main Author: BARTOSZ, M. SOSZYNSKI and G.
Format: Article
Language:English
Subjects:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - chemistry
Biological and medical sciences
Cell physiology
Chelating Agents - chemistry
Dithiothreitol - chemistry
Dose-Response Relationship, Radiation
Effects of physical and chemical agents
Erythrocytes - radiation effects
Fundamental and applied biological sciences. Psychology
Furosemide - chemistry
Glucose - chemistry
Hemolysis - radiation effects
Humans
Molecular and cellular biology
Osmolar Concentration
Spermine - chemistry
Valinomycin - chemistry
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Summary:The effect of postirradiation conditions on the haemolysis of gamma -irradiated (2.1 kGy) human erythrocytes was studied. Haemolysis was inhibited by incubation in mannitol and sucrose instead of saline, by hypertonicity of the medium and by calcium chelators. Dithiothreitol, butylated hydroxytoluene, deferoxamine, DIDS (an inhibitor of anion exchange) and furosemide (an inhibitor of K/Cl and K/Na/Cl cotransport) did not slow down the haemolysis. Apparently, the radiation-induced haemolysis is due to the formation of membrane pores leaky for electrolytes. From the inhibition of haemolysis by mannitol, apparent pore radius was estimated to be about 0.7 nm. The pores appear to be transient, the average pore number per cell being much less than unity.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530097144229