Exposure to ionizing radiation modifies neurally-evoked electrolyte transport and some inflammatory responses in rat colon in vitro

Purpose: To characterize the responsiveness of the colon to neural stimulation following acute exposure of rats to gamma -radiation and to correlate observed changes to a number of parameters. Materials and methods: Rats were exposed to 5 or 10 Gy 137Cs gamma radiation or not (sham-irradiated) and s...

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Bibliographic Details
Published in:International journal of radiation biology 1998, Vol.73 (1), p.93-101
Main Authors: FRANCOIS, A., AIGUEPERSE, J, GOURMELON, P, MACNAUGHTON, W. K., GRIFFITHS, N. M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carbachol - pharmacology
Cell physiology
Colon - metabolism
Colon - radiation effects
Effects of physical and chemical agents
Electric Stimulation
Electrolytes - metabolism
Fundamental and applied biological sciences. Psychology
Ion Transport
Leukotriene B4 - analysis
Male
Mast Cells - radiation effects
Molecular and cellular biology
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Rats
Rats, Wistar
Serotonin - analysis
Space life sciences
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Summary:Purpose: To characterize the responsiveness of the colon to neural stimulation following acute exposure of rats to gamma -radiation and to correlate observed changes to a number of parameters. Materials and methods: Rats were exposed to 5 or 10 Gy 137Cs gamma radiation or not (sham-irradiated) and studied at 1, 3 and 7 days after irradiation. Stripped segments of colon were mounted in Ussing chambers for measurements of neurally-evoked electrolyte transport (electrical field stimulation). Colonic tissue was also taken for biochemical (tissue 5-hydroxytryptamine, histamine, leukotriene B, nitric oxide synthase) and histological analyses 4 (mast cells). Results: In irradiated rats both proximal and distal colon were hyporesponsive to electrical field stimulation at 1 and 3 days, but had recovered by 7 days. In the distal colon, carbachol responses were attenuated 1 day after 10 Gy. Mast cells, tissue histamine and leukotriene B synthesis were significantly reduced 4 at all time points but no changes were seen in 5-HT or inducible NOS activity. Conclusions: Rat colon becomes hyporesponsive to neural stimuli post-irradiation. The response initially (1 and 3 days) correlates with decreased mast cells and histamine, but not at 7 days.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530098142743