Estimate of the frequency of true incomplete exchanges in human lymphocytes exposed to 1GeV/u Fe ions in vitro

Purpose: To study the frequency of true incomplete exchanges induced by high-LET radiation. Materials and methods: Human lymphocytes were exposed to 1GeV/u Fe ions (LET 140keV/ mu m). Chromosome aberrations were analysed by a fluorescence in situ hybridization using a combination of whole-chromosome...

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Bibliographic Details
Published in:International journal of radiation biology 1999, Vol.75 (5), p.593-599
Main Author: YANG, K. GEORGE, T. C.
Format: Article
Language:English
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Summary:Purpose: To study the frequency of true incomplete exchanges induced by high-LET radiation. Materials and methods: Human lymphocytes were exposed to 1GeV/u Fe ions (LET 140keV/ mu m). Chromosome aberrations were analysed by a fluorescence in situ hybridization using a combination of whole-chromosome-specific probes and human telomere probes. Chromosomes 1, 3 and 4 were investigated. Results: The percentage of incomplete exchanges was between 23 and 29% if telomere signals were not considered. The percentage decreased to 10% after ruling out false incomplete exchanges containing telomere signals. The final estimation of true incomplete exchanges was 10%. Conclusion: Within a degree of uncertainty, the percentage of true incomplete exchanges in 1GeV/u Fe ion-irradiated human lymphocytes was similar to that induced by gamma rays.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530099140230