Morphology and phylogenetic position of a trebouxiophycean green alga (Chlorophyta) growing on the rubber tree, Hevea brasiliensis , with the description of a new genus and species

A novel unicellular Chlorella-like green alga was isolated from the rubber tree (Hevea brasiliensis). The cultured cells were described by light and electron microscopy. Most adult cells were spherical with a single cup-shaped parietal chloroplast, although a few cells contained 2 to 4 chloroplasts....

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Bibliographic Details
Published in:European journal of phycology 2008-05, Vol.43 (2), p.185-193
Main Authors: Zhang, Jiaming, Huss, Volker A. R., Sun, Xuepiao, Chang, Kaijun, Pang, Daobiao
Format: Article
Language:English
Subjects:
Chlorella
Chlorella luteoviridis
Chlorella saccharophila
Chlorophyta
chloroplast
double hairpin element
Hevea brasiliensis
phylogeny
pyrenoid
taxonomy
Trebouxiophyceae
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Summary:A novel unicellular Chlorella-like green alga was isolated from the rubber tree (Hevea brasiliensis). The cultured cells were described by light and electron microscopy. Most adult cells were spherical with a single cup-shaped parietal chloroplast, although a few cells contained 2 to 4 chloroplasts. Chloroplasts contained many small pyrenoids with pyrenoglobuli around the multi-oriented tube-like thylakoids in the pyrenoid matrix. Most sporangia contained 2 or 4 autospores with a spindle-shaped chloroplast. Phylogenetic analyses inferred from both chloroplast-encoded 16S and nuclear-encoded 18S rDNA sequences placed our isolate in the 'Chlorella' luteoviridis clade within the class Trebouxiophyceae. There are two group I introns in the 18S rDNA, structurally similar to 'Chlorella' trebouxioides, which also has two introns at the same positions. The exon sequence revealed highest similarities of 93-95% compared with 'C.' luteoviridis, 'Chlorella' saccharophila, and 'C.' trebouxioides. The chloroplast 16S rDNA has a unique 46-bp insertion in helix P21-1, which can be folded into a double hairpin structure. Blast searches resulted in best hits of 96% similarity to 'C.' luteoviridis (CCAP-211/3), and of 91% to 'C.' saccharophila (SAG 211-1d). Based on morphological features and phylogenetic position, a new genus Heveochlorella gen. nov. with the type species H. hainangensis sp. nov. is proposed.
ISSN:0967-0262
1469-4433
DOI:10.1080/09670260701718462