Association of heterogenicity of Helicobacter pylori cag pathogenicity island with peptic ulcer diseases and gastric cancer

Objective: To investigate the frequency and integrity of certain cag pathogenicity island genes (cagPAI) in Helicobacter pylori strains and their association with peptic ulcer disease (PUD) and gastric cancer. Material and Methods: We enrolled 240 adult patients [120 with functional dyspepsia (FD),...

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Bibliographic Details
Published in:British journal of biomedical science 2017-07, Vol.74 (3), p.121-126
Main Authors: Khatoon, J., Prasad, K. N., Prakash Rai, R., Ghoshal, U. C., Krishnani, N.
Format: Article
Language:English
Subjects:
cagPAI
Dyspepsia
Endoscopy
functional dyspepsia
Gastric cancer
gastric tissues
Gene frequency
H. pylori
Health risk assessment
Helicobacter pylori
Pathogenicity
Pathogens
peptic ulcer diseases
Polymerase chain reaction
Primers
Trinucleotide repeats
Ulcers
Urea
Urease
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Summary:Objective: To investigate the frequency and integrity of certain cag pathogenicity island genes (cagPAI) in Helicobacter pylori strains and their association with peptic ulcer disease (PUD) and gastric cancer. Material and Methods: We enrolled 240 adult patients [120 with functional dyspepsia (FD), 50 with PUD and 70 with gastric cancer] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed when either culture or any two of the three tests (rapid urease test, histopathology and specific ureA PCR) were positive. DNA extracted from H. pylori isolates and positive gastric tissues were tested by PCR for the presence of different genes of cagPAI using specific primers. Results: A total of 122 (51%) patients were H. pylori positive. Frequencies of cagPAI genes cagA, cagE, cagT and cagM in H. pylori strains from different groups of patients were as follows: functional dyspepsia 73, 83, 76 and 60%, PUD 70, 94, 91, 70% and gastric cancer 75, 95, 90 and 70%, respectively. Risk associated for the presence of PUD and gastric cancer with cagPAI genes cagE, cagT and cagM was 5.0-, 4.6- and 4.1- and 3.0-, 2.8- and 2.5-folds, respectively. Prevalence of intact cagPAI was significantly higher in PUD and gastric cancer compared to functional dyspepsia (PUD vs. functional dyspepsia, 71% vs. 38%, P = 0.01; gastric cancer vs. functional dyspepsia, 75% vs. 38%, P 
ISSN:0967-4845
2474-0896
DOI:10.1080/09674845.2017.1278887