Metal-organic framework based on iron and terephthalic acid as a multiporous support for lipase Burkholderia lata LBBIO-BL02 and its potential for biocatalysis

• Published in: Biocatalysis and biotransformation 2023-09, Vol.41 (5), p.332-343
• Main Authors: Baron, Alessandra Machado, Rodrigues, Ricardo de Sousa, Sante, Luis Guilherme Giannina, Kister, Jocácia Muriele de Miranda, do Nascimento, Valéria Marta Gomes, Bail, Alesandro
• Format: Article
• Language: English
• Subjects: biocatalysis; hydrolysis; immobilization; Lipases; metal-organic frameworks
• ISSN: 1024-2422; 1029-2446
• DOI: 10.1080/10242422.2022.2068371

Metal-organic frameworks (MOFs) are versatile materials because they have a large internal surface area and tuneable pores, making them suitable for enzyme immobilization. In this study, we prepared a typical microporous Fe-BDC MOF through a thermal treatment to produce additional meso and macropores interconnected to each other, capable of immobilizing the Burkholderia lata LBBIO-BL02 (BLL) lipase by entrapment and physical adsorption. The immobilization efficiency (E) was 90%, and the activity retention (R) was 400% (pNPP hydrolysis). The immobilized lipase (BLL@BDC) also showed excellent activity in the hydrolysis of vegetable oils in aqueous medium, achieving up to 3,200 U g −1 for olive oil, as well as high stability in organic solvents, especially for polar ones, such as iso-propanol (101.5 ± 2.6%), ethanol (103.0 ± 6.0%) and acetone (107.7 ± 8.3%). The results indicate that the multiporous Fe-BDC MOF is a promising support for lipase immobilization and further application in biocatalysis performed in organic media.