Immobilization of Trametes trogii laccase on polyvinylpyrrolidone-coated magnetic nanoparticles for biocatalytic degradation of textile dyes

• Published in: Biocatalysis and biotransformation 2024-03, Vol.ahead-of-print (ahead-of-print), p.1-18
• Main Authors: Bakar, Büşra, Birhanlı, Emre, Ulu, Ahmet, Boran, Filiz, Yeşilada, Özfer, Ateş, Burhan
• Format: Article
• Language: English
• Subjects: dye decolorization; Indigo Carmine; laccase immobilization; Magnetic nanoparticles; polyvinylpyrrolidone; Remazol Brilliant Blue R
• ISSN: 1024-2422; 1029-2446
• DOI: 10.1080/10242422.2023.2173006

High cost and low operational stability are the most important challenges limiting the possible use of laccase in the removal of textile dyes. To overcome these challenges, in this study, polyvinylpyrrolidone (PVP)-coated magnetic nanoparticles (MNPs) were produced and characterized. To our knowledge, this is the first study to explore the feasibility of immobilizing a Trametes trogii laccase enzyme on Fe 3 O 4 /PVP MNPs. The characterization of samples and the successful immobilization of laccase were verified by characterization methods. Besides, the biochemical properties and stability of the Fe 3 O 4 /PVP/Lac were evaluated in terms of optimum pH, optimum temperature, thermostability, thermodynamic and kinetic parameters, storage stability, operational stability, and decolorization efficiency of two different textile dyes. The optimum activities were recorded at pH 2.5 °C and 30 °C. The Fe 3 O 4 /PVP/Lac displayed remarkable thermal stability and activation energy for denaturation, enthalpy, Gibbs free energy, and entropy results confirmed the enhanced stability of Fe 3 O 4 /PVP/Lac against high temperatures. Meanwhile, the Fe 3 O 4 /PVP/Lac retained about 58% of its original activity after seven consecutive reuses, while it retained up to 25% of its original activity after 28 d of storage at room temperature. K m and V max for the Fe 3 O 4 /PVP/Lac were calculated to be 126 µM and 211 µmol/min, respectively. Finally, after 8 and 6 cycles of repeated use, the Fe 3 O 4 /PVP/Lac still decolorized 32.34% and 32.23% of Remazol Brilliant Blue R (RBBR) and Indigo Carmine (IC), respectively. As envisioned, this study suggests a promising way to solve the problems of high price and poor operational stability of the enzyme during biocatalytic decolorization of textile dyes in wastewaters.