Expression of endocannabinoid synthetic enzyme mRNAs is correlated with cannabinoid 1 receptor mRNA in the mouse brain

The two principal endocannabinoids, N-arachidonoyl-ethanolamine (anandamide) and 2-arachidonoyl-glycerol (2-AG), are synthesized from arachidonic acid (AA) and AA is released as they are degraded. Therefore, the function of endocannabinoids is closely linked to AA, but the exact relationships have n...

Full description

Saved in:
Bibliographic Details
Published in:Nutritional neuroscience 2007-02, Vol.10 (1-2), p.45-50
Main Authors: Tsuyama, Shoichiro, Oikawa, Daichi, Yamasaki, Yasuko, Takagi, Sayuri, Ando, Hironori, Furuse, Mitsuhiro
Format: Article
Language:English
Subjects:
2-AG
Amidohydrolases - genetics
ANANDAMIDE
Animals
ARACHIDONIC ACID
Brain - enzymology
Brain - physiology
Cannabinoid Receptor Modulators - genetics
DNA Primers
ENDOCANNABINOID
Endocannabinoids
Gene Expression Regulation
Lipoprotein Lipase - genetics
Male
Mice
Mice, Inbred ICR
Phospholipase D - genetics
REAL-TIME RT-PCR
Receptor, Cannabinoid, CB1 - genetics
Receptors, Cannabinoid - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The two principal endocannabinoids, N-arachidonoyl-ethanolamine (anandamide) and 2-arachidonoyl-glycerol (2-AG), are synthesized from arachidonic acid (AA) and AA is released as they are degraded. Therefore, the function of endocannabinoids is closely linked to AA, but the exact relationships have not been clarified, especially with respect to endocannabinoid metabolism. In the present study, oil rich in AA was administered (0, 100, 200 and 300 m l) orally to male mice for 7 days. Phospholipase D (PLD), fatty acid amide hydrolase (FAAH), diacyl-glycerol lipase (DAGL), monoacyl-glycerol lipase (MAGL) and cannabinoid 1 (CB1) receptor mRNA expressions were determined in the whole brain. No changes in the expression of any gene investigated were detected following AA treatment. However, it was demonstrated that the expression of the CB1 receptor was positively correlated with PLD, FAAH and DAGL expression. This suggests that expression of the CB1 receptor is closely coordinated with that of the enzymes which synthesize its ligands.
ISSN:1028-415X
1476-8305
DOI:10.1080/10284150701250671