α-Glucosidase and lipoxygenase inhibitory derivatives of cryptosporioptide from the endophytic fungus Cryptosporiopsis sp

• Published in: Journal of asian natural products research 2014-11, Vol.16 (11), p.1068-1073
• Main Authors: Tousif, Muhammad Imran, Shazmeen, Natasha, Riaz, Naheed, Shafiq, Nusrat, Khatoon, Tasneem, Schulz, Barbara, Ashraf, Muhammad, Shaukat, Ayesha, Hussain, Hidayat, Jabbar, Abdul, Saleem, Muhammad
• Format: Article
• Language: English
• Subjects: Algorithms; alpha-glucosidase; alpha-Glucosidases - drug effects; Ascomycota - chemistry; carbon; Cryptosporiopsis; Cryptosporiopsis fungus; cryptosporioptide derivatives; endophytes; enzyme inhibition; fungi; Inhibitory Concentration 50; isolation; lipoxygenase; Lipoxygenase - drug effects; Models, Molecular; Molecular Structure; normal values; nuclear magnetic resonance spectroscopy; Nuclear Magnetic Resonance, Biomolecular; polyketides; Polyketides - chemistry; Polyketides - isolation & purification; Polyketides - pharmacology; shrubs; spectral analysis; stable isotopes; stereochemistry; structure elucidation; Viburnum - microbiology; Viburnum tinus
• ISSN: 1477-2213; 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2014.938647

Two new cryptosporioptide-derived polyketides cryptosporioptides A (2) and B (3) were isolated from the extract of endophytic fungus Cryptosporiopsis sp. associated with the shrub, Viburnum tinus . The structures of the isolates were determined through spectral analysis including 1D NMR (¹H, ¹³C) and 2D NMR (HSQC, HMBC, COSY) techniques, HR-FAB-MS and by comparison with the reported data of cryptosporioptide (1). The relative stereochemistry was assigned with the help of NOESY analysis, the molecular model, and comparison of the optical rotation values with the reference compound 1 .