Challenges and opportunities for circRNA identification and delivery

Circular RNAs (circRNAs) are evolutionarily conserved noncoding RNAs with tissue-specific expression patterns, and exert unique cellular functions that have the potential to become biomarkers in therapeutic applications. Therefore, accurate and sensitive detection of circRNA with facile platforms is...

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Bibliographic Details
Published in:Critical reviews in biochemistry and molecular biology 2023-01, Vol.58 (1), p.19-35
Main Authors: Dong, Jiani, Zeng, Zhuoer, Huang, Ying, Chen, Chuanpin, Cheng, Zeneng, Zhu, Qubo
Format: Article
Language:English
Subjects:
analysis
Biomarkers
biosensors
Circular RNA
detection
identification
RNA - genetics
RNA - metabolism
RNA, Circular
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Summary:Circular RNAs (circRNAs) are evolutionarily conserved noncoding RNAs with tissue-specific expression patterns, and exert unique cellular functions that have the potential to become biomarkers in therapeutic applications. Therefore, accurate and sensitive detection of circRNA with facile platforms is essential for better understanding of circRNA biological processes and circRNA-related disease diagnosis and prognosis; and precise regulation of circRNA through efficient delivery of circRNA or siRNA is critical for therapeutic purposes. Here, we reviewed the current development of circRNA identification methodologies, including overviewing the purification steps, summarizing the sequencing methods of circRNA, as well as comparing the advantages and disadvantages of traditional and new detection methods. Then, we discussed the delivery and manipulation strategies for circRNAs in both research and clinic treatment. Finally, the challenges and opportunities of analyzing circRNAs were addressed.
ISSN:1040-9238
1549-7798
DOI:10.1080/10409238.2023.2185764