Synthesis and Properties of Carbocyclic 5′- Nor Oligodeoxynucleotides as Potential Antisense Molecules

We have synthesized carbocyclic 5'-nor oligodeoxynucleotides with a shortened overall chain length lacking the 5'-methylene. Their hybridization properties with DNA and RNA were investigated by UV and CD melting curves. These oligomers formed unstable duplexes with DNA, but formed stable d...

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Bibliographic Details
Published in:Phosphorus, sulfur, and silicon and the related elements sulfur, and silicon and the related elements, 2002-08, Vol.177 (8-9), p.2099-2100
Main Authors: Koga, Masakazu, Tamai, Koichi, Ueda, Mitsunori, Uchida, Toshinori, Yamamuro, Toshiyuki, Shigeta, Masahiro, Yamamoto, Masaharu, Suzuki, Takaaki, Saeki, Toshiaki
Format: Article
Language:English
Subjects:
Carbocyclic 5′- nor Oligodeoxynucleotide
Hybridization Property
Nuclease Resistance
Rna Selectivity
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Summary:We have synthesized carbocyclic 5'-nor oligodeoxynucleotides with a shortened overall chain length lacking the 5'-methylene. Their hybridization properties with DNA and RNA were investigated by UV and CD melting curves. These oligomers formed unstable duplexes with DNA, but formed stable duplexes with RNA selectively. In addition, these oligomers were very stable against nucleases.
ISSN:1042-6507
1563-5325
DOI:10.1080/10426500213320