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Augmentation of hormonal activities with antibodies from cattle immunized with a combination of synthetic and recombinant growth hormone peptide

Antibodies generated against a synthetic growth hormone (GH) peptide in a number of animal species were shown to enhance the efficacy of GH. However, the ability to produce the effective antibodies diminished over time and repeated boosters failed to overcome the hurdle. Therefore, this study was de...

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Published in:Animal biotechnology 1998-01, Vol.9 (2), p.121-133
Main Authors: Wang, B.S. (Cyanamid Agricultural Research Center, Princton, NJ.), Search, D.J, Lumanglas, A.A, Ingling, J, Corbett, M.J, Shieh, H.M, Kraft, L.A
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container_title Animal biotechnology
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creator Wang, B.S. (Cyanamid Agricultural Research Center, Princton, NJ.)
Search, D.J
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Kraft, L.A
description Antibodies generated against a synthetic growth hormone (GH) peptide in a number of animal species were shown to enhance the efficacy of GH. However, the ability to produce the effective antibodies diminished over time and repeated boosters failed to overcome the hurdle. Therefore, this study was designed to address the issue on the failed antibody responses by employing different GH peptide antigen preparations in cattle. Holstein steers were repeatedly immunized with a synthetic peptide corresponding to an amino acid sequence 54-95 of porcine GH (pGH). The peptide was conjugated to ovalbumin (OVA) as a carrier. Animals initially responded to the antigen well and elicited antibodies specific to the peptide. However, the 4th challenge with the same OVA-peptide antigen rendered animals unresponsive, resulting in a decline in antibody production. This unresponsiveness was overcome by switching the antigen at the 5th immunization from OVA-peptide to a recombinant peptide preparation which was composed of maltose binding protein (MBP) as a carrier. Antibodies generated in cattle after the 5th immunization recognized not only the pGH(54-95) peptide, but also bovine GH (bGH) and pGH. These antibodies were not immunoreactive with an unrelated control peptide. Hypophysectomized (hypox) rats were used for functional analysis and bGH was active in promoting the growth of these GH-deficient rats. The growth-promoting effect of bGH was significantly enhanced by mixing it with bovine anti-peptide antibodies prior to administration. Therefore, the present findings suggest that peptide 54-95 induces cattle to elicit antibodies capable of not only recognizing bGH but also augmenting the somatogenic effectiveness of bGH in hypox rats.
doi_str_mv 10.1080/10495399809525899
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(Cyanamid Agricultural Research Center, Princton, NJ.) ; Search, D.J ; Lumanglas, A.A ; Ingling, J ; Corbett, M.J ; Shieh, H.M ; Kraft, L.A</creator><creatorcontrib>Wang, B.S. (Cyanamid Agricultural Research Center, Princton, NJ.) ; Search, D.J ; Lumanglas, A.A ; Ingling, J ; Corbett, M.J ; Shieh, H.M ; Kraft, L.A</creatorcontrib><description>Antibodies generated against a synthetic growth hormone (GH) peptide in a number of animal species were shown to enhance the efficacy of GH. However, the ability to produce the effective antibodies diminished over time and repeated boosters failed to overcome the hurdle. Therefore, this study was designed to address the issue on the failed antibody responses by employing different GH peptide antigen preparations in cattle. Holstein steers were repeatedly immunized with a synthetic peptide corresponding to an amino acid sequence 54-95 of porcine GH (pGH). The peptide was conjugated to ovalbumin (OVA) as a carrier. Animals initially responded to the antigen well and elicited antibodies specific to the peptide. However, the 4th challenge with the same OVA-peptide antigen rendered animals unresponsive, resulting in a decline in antibody production. This unresponsiveness was overcome by switching the antigen at the 5th immunization from OVA-peptide to a recombinant peptide preparation which was composed of maltose binding protein (MBP) as a carrier. Antibodies generated in cattle after the 5th immunization recognized not only the pGH(54-95) peptide, but also bovine GH (bGH) and pGH. These antibodies were not immunoreactive with an unrelated control peptide. Hypophysectomized (hypox) rats were used for functional analysis and bGH was active in promoting the growth of these GH-deficient rats. The growth-promoting effect of bGH was significantly enhanced by mixing it with bovine anti-peptide antibodies prior to administration. 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Therefore, this study was designed to address the issue on the failed antibody responses by employing different GH peptide antigen preparations in cattle. Holstein steers were repeatedly immunized with a synthetic peptide corresponding to an amino acid sequence 54-95 of porcine GH (pGH). The peptide was conjugated to ovalbumin (OVA) as a carrier. Animals initially responded to the antigen well and elicited antibodies specific to the peptide. However, the 4th challenge with the same OVA-peptide antigen rendered animals unresponsive, resulting in a decline in antibody production. This unresponsiveness was overcome by switching the antigen at the 5th immunization from OVA-peptide to a recombinant peptide preparation which was composed of maltose binding protein (MBP) as a carrier. Antibodies generated in cattle after the 5th immunization recognized not only the pGH(54-95) peptide, but also bovine GH (bGH) and pGH. These antibodies were not immunoreactive with an unrelated control peptide. Hypophysectomized (hypox) rats were used for functional analysis and bGH was active in promoting the growth of these GH-deficient rats. The growth-promoting effect of bGH was significantly enhanced by mixing it with bovine anti-peptide antibodies prior to administration. Therefore, the present findings suggest that peptide 54-95 induces cattle to elicit antibodies capable of not only recognizing bGH but also augmenting the somatogenic effectiveness of bGH in hypox rats.</abstract><cop>England</cop><pub>Taylor &amp; Francis Group</pub><pmid>9713677</pmid><doi>10.1080/10495399809525899</doi><tpages>13</tpages></addata></record>
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identifier ISSN: 1049-5398
ispartof Animal biotechnology, 1998-01, Vol.9 (2), p.121-133
issn 1049-5398
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language eng
recordid cdi_crossref_primary_10_1080_10495399809525899
source Taylor and Francis Science and Technology Collection
subjects Animals
Antibody Formation - physiology
Antibody Specificity
BOVIN
CATTLE
Cattle - immunology
Cattle - metabolism
Female
GANADO BOVINO
Growth Hormone - immunology
Growth Hormone - pharmacology
IMMUNISATION
IMMUNIZATION
INMUNIZACION
Male
Ovalbumin
Peptide Fragments - chemical synthesis
Peptide Fragments - immunology
Pilot Projects
Random Allocation
Rats
Recombinant Proteins - immunology
Recombinant Proteins - pharmacology
SOMATOTROPIN
SOMATOTROPINA
SOMATOTROPINE
Vaccination - veterinary
Vaccines, Synthetic
title Augmentation of hormonal activities with antibodies from cattle immunized with a combination of synthetic and recombinant growth hormone peptide
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