Effect of Explant Source, Culture Media, and Growth Regulators on Callogenesis and Expression of Secondary Metabolites of Curcuma Longa

The effects of explant source, medium composition, and growth regulators were examined in callus lines of Curcuma longa. Callus cultures were induced from leaf sheath, leaf lamina, leaf base, apical buds, and lateral buds. The growth potential of established callus lines varied with a combination of...

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Bibliographic Details
Published in:Journal of herbs, spices & medicinal plants spices & medicinal plants, 2020-04, Vol.26 (2), p.172-190
Main Authors: Gurav, Shailendra S., Gurav, Nilambari S., Patil, Arun T., Duragkar, Nandkishore J.
Format: Article
Language:English
Subjects:
2,4-D
Antioxidants
Aquatic plants
Buds
Callus
callus induction
Chromatography
Culture media
Curcumin
Growth regulators
in-vitro culture
Kinetin
Leaves
Metabolites
MS medium
Oxidants
Oxidizing agents
Plant hormones
Polyphenols
Rhizomes
Secondary metabolites
Sheaths
Turmeric
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Summary:The effects of explant source, medium composition, and growth regulators were examined in callus lines of Curcuma longa. Callus cultures were induced from leaf sheath, leaf lamina, leaf base, apical buds, and lateral buds. The growth potential of established callus lines varied with a combination of phytohormones and culture medium. The greatest callus induction (79%) after 21 d was observed with MS medium supplemented with 3 mg L −1 2,4-D and 0.8 mg mL −1 kinetin. Phytochemical and chromatographic analysis of callus extract after 6th sub-culture revealed the presence of 53.6 mg g −1 of total polyphenols and 36.1 mg g −1 curcumin dry wt. with higher in-vitro anti-oxidant activity compared to turmeric rhizome extract. The lateral bud callus of C. longa may be used as a starting material for the synthesis of secondary metabolites of pharmaceutical interest.
ISSN:1049-6475
1540-3580
DOI:10.1080/10496475.2019.1689542