Differential expression of microRNA between triple negative breast cancer patients of African American and European American descent

There are racial disparities in the outcome of triple negative breast cancer (TNBC) patients between women of African ancestry and women of European ancestry, even after accounting for lifestyle, socioeconomic and clinical factors. MicroRNA (miRNA) are non-coding molecules whose level of expression...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2022, Vol.97 (1), p.1-10
Main Authors: MacCuaig, William M., Thomas, Alexandra, Claros-Sorto, Juan C., Gomez-Gutierrez, Jorge G., Alexander, Adam C., Wellberg, Elizabeth A., Grizzle, William E., McNally, Lacey R.
Format: Article
Language:English
Subjects:
African American
Black or African American - genetics
Caucasian
European American
Female
Gene Expression Regulation, Neoplastic
human
Humans
laser-capture microdissection
microRNA
MicroRNAs - genetics
racial disparity
triple negative breast cancer
Triple Negative Breast Neoplasms - genetics
White People - genetics
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Summary:There are racial disparities in the outcome of triple negative breast cancer (TNBC) patients between women of African ancestry and women of European ancestry, even after accounting for lifestyle, socioeconomic and clinical factors. MicroRNA (miRNA) are non-coding molecules whose level of expression is associated with cancer suppression, proliferation and drug resistance; therefore, these have potential for biomarker applications in cancers including TNBC. Historically, miRNAs up-regulated in African American (AA) patients have received less attention than for patients of European ancestry. Using laser capture microdissection (LCM) to acquire ultrapure tumor cell samples, miRNA expression was evaluated in 15 AA and 15 European American (EA) TNBC patients. Tumor sections were evaluated using RNA extraction followed by miRNA analysis and profiling. Results were compared based on ethnicity and method of tissue fixation. miRNAs that showed high differential expression in AA TNBC patients compared to EA included: miR-19a, miR-192, miR-302a, miR-302b, miR-302c, miR-335, miR-520b, miR-520f and miR-645. LCM is a useful technique for isolation of tumor cells. We found a greater abundance of RNA in frozen samples compared to formalin fixed, paraffin embedded samples. miRNA appears to be a useful biomarker for TNBC to improve diagnosis and treatment.
ISSN:1052-0295
1473-7760
DOI:10.1080/10520295.2021.2005147