The protective effect of alpha lipoic acid against traumatic brain injury in rats

Traumatic brain injury (TBI) was induced by a weight-drop device using 300 g-1 m weight-height impact. The study groups were: control, alpha-lipoic acid (LA) (100 mg/kg, po), TBI, and TBI + LA (100 mg/kg, po). Forty-eight hours after the injury, neurological scores were measured and brain samples we...

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Bibliographic Details
Published in:Free radical research 2009-01, Vol.43 (7), p.658-667
Main Authors: Toklu, Hale Z., Hakan, Tayfun, Bi ber, Necat, Solako lu, Seyhun, Ö ünç, Ayli z Velio lu, ener, Göksel
Format: Article
Language:English
Subjects:
Alpha lipoic acid
Animals
Antioxidants - pharmacology
Blood-Brain Barrier
Brain Edema - metabolism
Brain Edema - pathology
Brain Edema - prevention & control
Brain Injuries - metabolism
Brain Injuries - pathology
Brain Injuries - prevention & control
brain injury
closed head
Glutathione - metabolism
Interleukin-1beta - metabolism
Neuroprotective Agents - pharmacology
oedema
Oxidative Stress - drug effects
Peroxidase - metabolism
Rats
Rats, Wistar
Thiobarbituric Acid Reactive Substances - metabolism
Thioctic Acid - pharmacology
trauma
Tumor Necrosis Factor-alpha - metabolism
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Summary:Traumatic brain injury (TBI) was induced by a weight-drop device using 300 g-1 m weight-height impact. The study groups were: control, alpha-lipoic acid (LA) (100 mg/kg, po), TBI, and TBI + LA (100 mg/kg, po). Forty-eight hours after the injury, neurological scores were measured and brain samples were taken for histological examination or determination of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, myeloperoxidase (MPO) and Na+-K+ ATPase activities, whereas cytokines (TNF- , IL-1 ) were determined in blood. Brain oedema was evaluated by wet-dry weight method and blood-brain barrier (BBB) permeability was evaluated by Evans Blue (EB) extravasation. As a result, neurological scores mildly increased in trauma groups. Moreover, TBI caused a significant decrease in brain GSH and Na+-K+ ATPase activity, which was accompanied with significant increases in TBARS level, MPO activity and plasma proinflammatory cytokines. LA treatment reversed all these biochemical indices as well as histopathological alterations. TBI also caused a significant increase in brain water content and EB extravasation which were partially reversed by LA treatment. These findings suggest that LA exerts neuroprotection by preserving BBB permeability and by reducing brain oedema probably by its anti-inflammatory and antioxidant properties in the TBI model.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760902988843