Determination of anthocyanins and anthocyanidins in the wild grape (Vitis sylvestris Gmelin) by high-performance liquid chromatography-diode array detection (HPLC-DAD)

The determination of anthocyanins and anthocyanidins in wild grapes (Vitis sylvestris Gmelin) from Tunceli-Turkey are reported together with the influence of the extraction conditions on the results. Representative sets of anthocyanins (delphinidin-3-o-glucoside, cyanidin-3-o-glucoside, pelargonidin...

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Bibliographic Details
Published in:Instrumentation science & technology 2022-01, Vol.50 (1), p.57-68
Main Authors: Karaaslan-Ayhan, Nagihan, Yaman, Mehmet
Format: Article
Language:English
Subjects:
anthocyanidin
Anthocyanin
Anthocyanins
Arrays
Chromatography
extraction condition
Glucosides
Grapes
High performance liquid chromatography
high-performance liquid chromatography - diode array detection
Liquid chromatography
wild grape
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Summary:The determination of anthocyanins and anthocyanidins in wild grapes (Vitis sylvestris Gmelin) from Tunceli-Turkey are reported together with the influence of the extraction conditions on the results. Representative sets of anthocyanins (delphinidin-3-o-glucoside, cyanidin-3-o-glucoside, pelargonidin-3-o-glucoside, malvidin-3-o-glucoside) and anthocyanidins (delphinidin, cyanidin, pelargonidin, malvidin) were simultaneously determined by high performance liquid chromatography with diode array detection (HPLC-DAD). The extraction conditions including the solvent type, extraction time and liquid:solid ratio (v:w) were optimized. The anthocyanin and anthocyanidin contents changed significantly depending on the extraction conditions. Malvidin-3-o-glucoside was identified to be the primary compound.
ISSN:1073-9149
1525-6030
DOI:10.1080/10739149.2021.1954532