Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system

Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy othe...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2018-01, Vol.48 (8), p.759-767
Main Authors: Bommenahalli Shashidhara, Rashmi, Iyyaswami, Regupathi
Format: Article
Language:English
Subjects:
Affinity chromatography
Ammonium
Binary systems
Citric acid
Citric Acid - chemistry
Ethylene glycol
HIV
Human immunodeficiency virus
Ionic strength
Lectin
Lectins
Partitioning
partitioning coefficient
pH effects
phase diagram
Pisum sativum
Pisum sativum - chemistry
Plant Lectins - chemistry
Plant Lectins - isolation & purification
Polyethylene glycol
Polyethylene Glycols - chemistry
Potassium
Purification
RNA-directed DNA polymerase
Salts
Sodium
Sodium chloride
Sodium citrate
tie line length
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Summary:Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2018.1504220