Synergism of cellulase, pectinase and xylanase on hydrolyzing differently pretreated sweet potato residues

The synergism of cellulase (C), pectinase (P), and xylanase (X) for the saccharification of sweet potato residues (SPR) was investigated. The removal of starch from SPR was easily achieved by using amylase, but the cellulose conversion of de-starched SPR was relatively low, thus dilute H 2 SO 4 , Na...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2020-02, Vol.50 (2), p.181-190
Main Authors: Xia, Jun, Shu, Jingyi, Yao, Kangwei, Xu, Jiming, Yu, Xinjun, Xue, Xiang, Ma, Decao, Lin, Xueqi
Format: Article
Language:English
Subjects:
Cellulase
Cellulase - metabolism
Cellulose
Conversion
Digestibility
Endo-1,4-beta Xylanases - metabolism
Enzymes
Galactose
Glucose
Hemicellulose
Hydrogen peroxide
Hydrolysis
Ipomoea batatas - chemistry
Ipomoea batatas - metabolism
Lignin
Monosaccharides
Pectin
Pectinase
Polygalacturonase - metabolism
Potatoes
Pretreatment
Residues
Saccharification
Sodium hydroxide
Starch
Sulfuric acid
Sulfuric Acids - metabolism
sweet potato residues
Sweet potatoes
Synergism
Synergistic effect
Xylanase
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Summary:The synergism of cellulase (C), pectinase (P), and xylanase (X) for the saccharification of sweet potato residues (SPR) was investigated. The removal of starch from SPR was easily achieved by using amylase, but the cellulose conversion of de-starched SPR was relatively low, thus dilute H 2 SO 4 , NaOH, and H 2 O 2 pretreatment was conducted to improve the enzymatic digestibility. The lignin content of NaOH pretreated SPR was the lowest, whereas H 2 SO 4 pretreatment resulted in the lowest contents of hemicellulose and pectin. The combination of C, P, and X exhibited different sugar production patterns, C-P displayed synergistic action on glucose and galactose production from each type of SPR, C-X also exhibited synergistic effect on glucose production except when H 2 SO 4 pretreated SPR was used, whereas no synergism between P-X on monosaccharide production was observed. The presence of synergism between cellulase and mixed accessory enzymes [C-(PX)] on glucose formation was determined by C-X, and the degree of synergism between C-P and C-(PX) on glucose production had a positive relationship with pectin content. The highest cellulose conversion of 96.2% was obtained from NaOH pretreated SPR using mixed enzymes comprising C, P, and X with the ratio of 8:1:1.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2019.1680390