Production, purification and application of Cutinase in enzymatic scouring of cotton fabric isolated from Acinetobacter baumannii AU10

Conventional cotton scouring in the textile industry using alkali results in huge environmental impact which can be overcome by using enzymes. Pectinase along with cutinase gives enhanced bioscouring results. Cutin was extracted from tomato peels and was used as substrate in the microbial media. The...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2021-07, Vol.51 (6), p.550-561
Main Authors: Gururaj, P., Khushbu, S., Monisha, B., Selvakumar, N., Chakravarthy, M., Gautam, P., Nandhini Devi, G.
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Ammonium
Ammonium sulfate
Bioscouring
Chromatography
Cotton
Cotton fabrics
Cutin
Cutinase
Environmental impact
Gel electrophoresis
Gelatin
High performance liquid chromatography
hydrolase
Hydrophobicity
Ion exchange
Liquid chromatography
Microorganisms
Microscopic analysis
Pectinase
Placket-Burmann
Pollution control
Response surface methodology
Response Surface Methodology (RSM)
rRNA 16S
Scanning electron microscopy
Scouring
Sodium lauryl sulfate
Substrates
Sucrose
Textile industry
tomato peel
Tomatoes
Wetting
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Summary:Conventional cotton scouring in the textile industry using alkali results in huge environmental impact which can be overcome by using enzymes. Pectinase along with cutinase gives enhanced bioscouring results. Cutin was extracted from tomato peels and was used as substrate in the microbial media. The strain isolated from tomato peel was identified as Acinetobacter baumannii AU10 by 16S rDNA sequencing. The cutinase production was optimized by Placket-Burman and Response Surface Methodology (RSM) and the maximum production of 82.75 U/mL obtained at sucrose 6.68% (w/v), gelatin 2.74 g/L at a temperature of 35.93 °C. Cutinase was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatography with a recovery of 25.6% and specific activity of 38030 U/mg. The confirmation test for the purity of cutinase was analyzed by RP-HPLC. The molecular mass of cutinase was determined as 28.9 kDa by SDS-PAGE technique. Scanning electron microscopic analysis showed a rough and open primary wall surface on the cutinase bioscoured fabric which confirmed its activity on cutin present in the cotton fabric. Additionally, the cutinase-bioscoured samples showed better absorbency than the untreated samples. Therefore, enzymatic scouring increases wetting capacity of scoured cotton and also helps to reduce environmental pollution.
ISSN:1082-6068
1532-2297
DOI:10.1080/10826068.2020.1836655