Identification of Drugs by Ultra Fast Liquid Chromatography/Electrospray Ionization-Quadrupole Ion Trap/Time-of-Flight Mass Spectrometry

Hybrid quadrupole ion trap/time-of-flight mass spectrometry (QIT/TOFMS) was applied for detection of ultra fast liquid chromatography (UFLC). The system performance for fast qualitative analysis was evaluated using test drugs. The test drugs were separated on a C 18 column (30 mm×2.0 mm I.D., partic...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2008-01, Vol.31 (1), p.23-37
Main Authors: Kawano, Shin-ichi, Inohana, Yusuke, Arakawa, Kiyomi, Mikami, Hirohisa, Yamaguchi, Shin-ichi
Format: Article
Language:English
Subjects:
Drug
Formula prediction
Liquid chromatography
Mass accuracy
Tandem mass spectrometry
Time-of-flight mass spectrometry
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Summary:Hybrid quadrupole ion trap/time-of-flight mass spectrometry (QIT/TOFMS) was applied for detection of ultra fast liquid chromatography (UFLC). The system performance for fast qualitative analysis was evaluated using test drugs. The test drugs were separated on a C 18 column (30 mm×2.0 mm I.D., particle size: 2.2 µm) with fast linear gradient elution using 0.1% formic acid and acetonitrile as mobile phase. The flow rate was set at 0.5 mL/min and the analysis cycle time was 4.5 min. Relative standard deviations (RSDs) of retention time and peak area for each drug (200 pg each) were 0.2% or better, lower than 3%, respectively. Mass accuracy of each compound was found to be 3.2 ppm or better. Rapid positive to negative polarity switching mode was demonstrated, showing good mass accuracy below 5 ppm. Moreover, an MS 3 measurement was carried out and the formulae of compounds were confirmed using formula prediction software.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826070701665493