High performance thin layer chromatography fingerprint on cyano-bonded stationary phase of selected Stachys and Betonica species and their antioxidant activity with chemometric calculations

The methanolic extracts of selected Stachys and Betonica species were analyzed by high performance thin layer chromatography (HPTLC) method using cyano bonded stationary phase and mobile phase consisted of methanol and water 45:55 (v/v) with Natural product reagent derivatization. The HPTLC method w...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2024-06, Vol.47 (6-10), p.162-170
Main Authors: Hawrył, Anna, Hawrył, Mirosław, Kowaleczko, Aleksandra, Hawrył, Dominika, Chernetskyy, Mykhaylo
Format: Article
Language:English
Subjects:
Antioxidant activity
Antioxidants
Betonica
Chromatography
Cluster analysis
Correlation coefficient
Correlation coefficients
Ferric ions
Fingerprints
Gallic acid
HCA
HPTLC
Natural products
PCA
Phenolic compounds
Phenols
Plant extracts
Principal components analysis
quantitative analysis
Reagents
Similarity
Stachys
Stationary phase
Thin layer chromatography
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Summary:The methanolic extracts of selected Stachys and Betonica species were analyzed by high performance thin layer chromatography (HPTLC) method using cyano bonded stationary phase and mobile phase consisted of methanol and water 45:55 (v/v) with Natural product reagent derivatization. The HPTLC method was validated (linearity, precision, accuracy, robustness, LOD, LOQ) and the validation results confirm the usefulness of the method used. The obtained images of fingerprint chromatograms presented as numerical data (using the TLC analyzer program) were next processed using Excel and SpecAlign softwares. The phytochemical similarity between analyzed plant extracts was evaluated using the similarity index (Pearson correlation coefficient) and the results were presented as hierarchical cluster analysis dendrograms and as graph for principal component analysis (PCA). The total phenolic content (TPC) by Folin-Ciocalteu method and the antioxidant activity with Ferric Ion Reducing Antioxidant Potential (FRAP) technique were determined for studied extracts using gallic acid as standards in both methods.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2024.2336063