Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography

High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 1998-01, Vol.21 (1-2), p.143-155
Main Authors: Ikai, Y., Oka, H., Hayakawa, J., Kawamura, N., Harada, K., Suzuki, M., Nakazawa, H., Ito, Y.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
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Summary:High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90%. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826079808001943