Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography

High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 1998-01, Vol.21 (1-2), p.143-155
Main Authors: Ikai, Y., Oka, H., Hayakawa, J., Kawamura, N., Harada, K., Suzuki, M., Nakazawa, H., Ito, Y.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73
cites cdi_FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73
container_end_page 155
container_issue 1-2
container_start_page 143
container_title Journal of liquid chromatography & related technologies
container_volume 21
creator Ikai, Y.
Oka, H.
Hayakawa, J.
Kawamura, N.
Harada, K.
Suzuki, M.
Nakazawa, H.
Ito, Y.
description High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90%. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components.
doi_str_mv 10.1080/10826079808001943
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1080_10826079808001943</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16473617</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73</originalsourceid><addsrcrecordid>eNqFkM1LwzAUwIsoOKd_gLcexFs1n00LXuZQNxh40IG3kqbJFmmTmqTo_nszNr0M9PI-eL_fe_CS5BKCGwgKcBsDygEri9gAWBJ8lIwgRSCjDL0dxzrOswjkp8mZ9--RYRTRUbKYe9vyoK1JrUqnttU-aJNOUm6a9D5dem1W6Uyv1tlLL2UTicEE6cTgnDQhna6d7XiwK8f79eY8OVG89fJin8fJ8vHhdTrLFs9P8-lkkQkCSMhgIUXOKKxJrnKMpAJc4qbGuUBQyJoUjBWSEgxqgiSgEFHRFIrSklFAmGJ4nFzv9vbOfgzSh6rTXsi25UbawVcwJwzncAvCHSic9d5JVfVOd9xtKgiq7d-qg79F52q_nHvBW-W4Edr_igiWGAMYsbsdpo2yruOf1rVNFfimte7HwX9dYf_qB1YVvgL-BgBRkOY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16473617</pqid></control><display><type>article</type><title>Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography</title><source>Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list)</source><creator>Ikai, Y. ; Oka, H. ; Hayakawa, J. ; Kawamura, N. ; Harada, K. ; Suzuki, M. ; Nakazawa, H. ; Ito, Y.</creator><creatorcontrib>Ikai, Y. ; Oka, H. ; Hayakawa, J. ; Kawamura, N. ; Harada, K. ; Suzuki, M. ; Nakazawa, H. ; Ito, Y.</creatorcontrib><description>High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90%. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components.</description><identifier>ISSN: 1082-6076</identifier><identifier>EISSN: 1520-572X</identifier><identifier>DOI: 10.1080/10826079808001943</identifier><identifier>CODEN: JLCTFC</identifier><language>eng</language><publisher>Colchester: Taylor &amp; Francis Group</publisher><subject>Analysis ; Biological and medical sciences ; General pharmacology ; Medical sciences ; Pharmacology. Drug treatments</subject><ispartof>Journal of liquid chromatography &amp; related technologies, 1998-01, Vol.21 (1-2), p.143-155</ispartof><rights>Copyright Taylor &amp; Francis Group, LLC 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73</citedby><cites>FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2193301$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikai, Y.</creatorcontrib><creatorcontrib>Oka, H.</creatorcontrib><creatorcontrib>Hayakawa, J.</creatorcontrib><creatorcontrib>Kawamura, N.</creatorcontrib><creatorcontrib>Harada, K.</creatorcontrib><creatorcontrib>Suzuki, M.</creatorcontrib><creatorcontrib>Nakazawa, H.</creatorcontrib><creatorcontrib>Ito, Y.</creatorcontrib><title>Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography</title><title>Journal of liquid chromatography &amp; related technologies</title><description>High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90%. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><issn>1082-6076</issn><issn>1520-572X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkM1LwzAUwIsoOKd_gLcexFs1n00LXuZQNxh40IG3kqbJFmmTmqTo_nszNr0M9PI-eL_fe_CS5BKCGwgKcBsDygEri9gAWBJ8lIwgRSCjDL0dxzrOswjkp8mZ9--RYRTRUbKYe9vyoK1JrUqnttU-aJNOUm6a9D5dem1W6Uyv1tlLL2UTicEE6cTgnDQhna6d7XiwK8f79eY8OVG89fJin8fJ8vHhdTrLFs9P8-lkkQkCSMhgIUXOKKxJrnKMpAJc4qbGuUBQyJoUjBWSEgxqgiSgEFHRFIrSklFAmGJ4nFzv9vbOfgzSh6rTXsi25UbawVcwJwzncAvCHSic9d5JVfVOd9xtKgiq7d-qg79F52q_nHvBW-W4Edr_igiWGAMYsbsdpo2yruOf1rVNFfimte7HwX9dYf_qB1YVvgL-BgBRkOY</recordid><startdate>19980101</startdate><enddate>19980101</enddate><creator>Ikai, Y.</creator><creator>Oka, H.</creator><creator>Hayakawa, J.</creator><creator>Kawamura, N.</creator><creator>Harada, K.</creator><creator>Suzuki, M.</creator><creator>Nakazawa, H.</creator><creator>Ito, Y.</creator><general>Taylor &amp; Francis Group</general><general>Taylor &amp; Francis</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19980101</creationdate><title>Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography</title><author>Ikai, Y. ; Oka, H. ; Hayakawa, J. ; Kawamura, N. ; Harada, K. ; Suzuki, M. ; Nakazawa, H. ; Ito, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikai, Y.</creatorcontrib><creatorcontrib>Oka, H.</creatorcontrib><creatorcontrib>Hayakawa, J.</creatorcontrib><creatorcontrib>Kawamura, N.</creatorcontrib><creatorcontrib>Harada, K.</creatorcontrib><creatorcontrib>Suzuki, M.</creatorcontrib><creatorcontrib>Nakazawa, H.</creatorcontrib><creatorcontrib>Ito, Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of liquid chromatography &amp; related technologies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikai, Y.</au><au>Oka, H.</au><au>Hayakawa, J.</au><au>Kawamura, N.</au><au>Harada, K.</au><au>Suzuki, M.</au><au>Nakazawa, H.</au><au>Ito, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography</atitle><jtitle>Journal of liquid chromatography &amp; related technologies</jtitle><date>1998-01-01</date><risdate>1998</risdate><volume>21</volume><issue>1-2</issue><spage>143</spage><epage>155</epage><pages>143-155</pages><issn>1082-6076</issn><eissn>1520-572X</eissn><coden>JLCTFC</coden><abstract>High-speed countercurrent chromatography was successfully applied to the isolation of colistin-A and colistin-B from a commercial colistin preparation. As the first step. TLC and HPLC analysis conditions for the colistin components were established. Using these techniques, a two-phase solvent system composed of n-butanol-0.04M aqueous trifluoroacetic acid (TFA) (1:1) was selected for high-speed CCC, where the concentration of TFA was the major factor in controlling the partition coefficients of the colistin components. Yields of colistin-A and colistin-B were 9 mg each from 20 mg of the commercial sample, and the purity of each component was over 90%. Fast atom bombardment (FAB) mass spectrometry was utilized to confirm the nature of the isolated components.</abstract><cop>Colchester</cop><pub>Taylor &amp; Francis Group</pub><doi>10.1080/10826079808001943</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1082-6076
ispartof Journal of liquid chromatography & related technologies, 1998-01, Vol.21 (1-2), p.143-155
issn 1082-6076
1520-572X
language eng
recordid cdi_crossref_primary_10_1080_10826079808001943
source Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list)
subjects Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
title Isolation of Colistin A and B Using High-Speed Countercurrent Chromatography
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T23%3A48%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20of%20Colistin%20A%20and%20B%20Using%20High-Speed%20Countercurrent%20Chromatography&rft.jtitle=Journal%20of%20liquid%20chromatography%20&%20related%20technologies&rft.au=Ikai,%20Y.&rft.date=1998-01-01&rft.volume=21&rft.issue=1-2&rft.spage=143&rft.epage=155&rft.pages=143-155&rft.issn=1082-6076&rft.eissn=1520-572X&rft.coden=JLCTFC&rft_id=info:doi/10.1080/10826079808001943&rft_dat=%3Cproquest_cross%3E16473617%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c404t-18ec6751b46f632ef0ae3db36c21ceb48778e5430b42e05125cd8f55975047f73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16473617&rft_id=info:pmid/&rfr_iscdi=true