Cloning and Characterization of a Novel Hsp100/Clp Gene (osClpD) from Oryza sativa

A novel osClpD gene, encoding a highly conservative ClpD subfamily member, was first isolated and characterized from Oryza sativa. The full-length cDNA of osClpD gene was 3140 bp and contained a 2884 bp open reading frame encoding a 938 amino acid protein. The phylogenetic tree and blast search show...

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Bibliographic Details
Published in:DNA sequence 2003-07, Vol.14 (4), p.285-293
Main Authors: Shen, Guo-an, Pang, Yong-zhen, Lin, Chang-fa, Wei, Chun, Qian, Xiao-yin, Jiang, Li-zhi, Du, Xi-ling, Li, Ke-gui, ATTia, Kotb, Yang, Jin-shui
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Amino Acid Sequence
AY166599.1
Base Sequence
Cluster Analysis
Desiccation
DNA Primers
DNA, Complementary - genetics
Endopeptidase Clp
Hsp100/Clp
Molecular Sequence Data
mRNA
Oryza - genetics
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Serine Endopeptidases - genetics
Temperature
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Summary:A novel osClpD gene, encoding a highly conservative ClpD subfamily member, was first isolated and characterized from Oryza sativa. The full-length cDNA of osClpD gene was 3140 bp and contained a 2884 bp open reading frame encoding a 938 amino acid protein. The phylogenetic tree and blast search showed that OSClpD belonged to the ClpD subfamily of the Hsp100/Clp family, and contained all protein motifs characteristic for the ClpD subfamily of Hsp100/Clp proteins. The real-time quantitative PCR analysis proved that it was inducible by water deficit and temperature stress in vegetative tissues.
ISSN:1042-5179
1029-2365
DOI:10.1080/1085566031000141153